PXD020140 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discovery of human breast cancer cell MCF7 heat induced proteins by LC-MS/MS |
Description | Co-translational degradation via the ubiquitin-proteasome system mediates quality control of 15 – 25% of nascent proteins, a proportion that is known to increase dramatically under proteotoxic stress conditions. Whereas the ubiquitylation machinery involved has been characterized, mechanisms coordinating the proteasomal destruction of ribosome-attached nascent proteins remain poorly defined. In pursuit of such mechanisms, we discovered dual cooperation of the HSP70 family member HSPA1 with the 26S proteasome: First, in response to proteotoxic stress, HSPA1 promotes proteasome recruitment to translating 80S ribosomes in a manner independent of nascent chain ubiquitylation. Secondly, HSPA1, in association with its cognate nucleotide exchange factor HSPH1, maintains co-translationally ubiquitylated proteins in a soluble state required for efficient proteasomal degradation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:28:30.019.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guiyou Tian |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-01 08:53:22 | ID requested | |
1 | 2021-09-09 02:17:23 | announced | |
⏵ 2 | 2024-10-22 05:28:37 | announced | 2024-10-22: Updated project metadata. |
Publication List
Tian G, Hu C, Yun Y, Yang W, Dubiel W, Cheng Y, Wolf DA, Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins. EMBO J, 40(13):e106183(2021) [pubmed] |
10.15252/embj.2020106183; |
Keyword List
ProteomeXchange project tag: Protein Misfolding and Aggregation (B/D-HPP), Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: chaperones,Heat shock response, proteasome, LC-MS/MS |
Contact List
Dieter A.Wolf |
contact affiliation | School of Pharmaceutical Sciences, Fujian Provincial Key Laboratory of Innovative Drug Target Research and Center for Stress Signaling Networks, Xiamen University, Xiamen, Fujian 361102, China |
contact email | dwolf@xmu.edu.cn |
lab head | |
Guiyou Tian |
contact affiliation | School of Pharmaceutical Sciences, Fujian Provincial Key Laboratory of Innovative Drug Target Research and Center for Stress Signaling Networks, Xiamen University, Xiamen, Fujian 361102, China |
contact email | tian19880912@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020140
- Label: PRIDE project
- Name: Discovery of human breast cancer cell MCF7 heat induced proteins by LC-MS/MS