PXD020140

PXD020140 is an original dataset announced via ProteomeXchange.

Title	Discovery of human breast cancer cell MCF7 heat induced proteins by LC-MS/MS
Description	Co-translational degradation via the ubiquitin-proteasome system mediates quality control of 15 – 25% of nascent proteins, a proportion that is known to increase dramatically under proteotoxic stress conditions. Whereas the ubiquitylation machinery involved has been characterized, mechanisms coordinating the proteasomal destruction of ribosome-attached nascent proteins remain poorly defined. In pursuit of such mechanisms, we discovered dual cooperation of the HSP70 family member HSPA1 with the 26S proteasome: First, in response to proteotoxic stress, HSPA1 promotes proteasome recruitment to translating 80S ribosomes in a manner independent of nascent chain ubiquitylation. Secondly, HSPA1, in association with its cognate nucleotide exchange factor HSPH1, maintains co-translationally ubiquitylated proteins in a soluble state required for efficient proteasomal degradation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:30.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guiyou Tian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-01 08:53:22	ID requested
1	2021-09-09 02:17:23	announced
⏵ 2	2024-10-22 05:28:37	announced	2024-10-22: Updated project metadata.

Tian G, Hu C, Yun Y, Yang W, Dubiel W, Cheng Y, Wolf DA, Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins. EMBO J, 40(13):e106183(2021) [pubmed]

10.15252/embj.2020106183;

ProteomeXchange project tag: Protein Misfolding and Aggregation (B/D-HPP), Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: chaperones,Heat shock response, proteasome, LC-MS/MS

Dieter A.Wolf
contact affiliation	School of Pharmaceutical Sciences, Fujian Provincial Key Laboratory of Innovative Drug Target Research and Center for Stress Signaling Networks, Xiamen University, Xiamen, Fujian 361102, China
contact email	dwolf@xmu.edu.cn
lab head
Guiyou Tian
contact affiliation	School of Pharmaceutical Sciences, Fujian Provincial Key Laboratory of Innovative Drug Target Research and Center for Stress Signaling Networks, Xiamen University, Xiamen, Fujian 361102, China
contact email	tian19880912@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020140

PRIDE project URI

• PRIDE
  1. PXD020140
     1. Label: PRIDE project
     2. Name: Discovery of human breast cancer cell MCF7 heat induced proteins by LC-MS/MS