PXD020138

PXD020138 is an original dataset announced via ProteomeXchange.

Title	Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce.
Description	Photosystem II (PSII) complexes are organized into large supercomplexes with variable amounts of light-harvesting proteins (Lhcb). A typical PSII supercomplex in plants is formed by four trimers of Lhcb proteins (LHCII trimers), which are bound to the PSII core dimer via monomeric antenna proteins. However, the architecture of PSII supercomplexes in Norway spruce (Picea abies (L.) Karst.) is different, most likely due to a lack of two Lhcb proteins, Lhcb6 and Lhcb3. Interestingly, the spruce PSII supercomplex shares similar structural features with its counterpart in the green alga Chlamydomonas reinhardtii (Kouřil et al.: New Phytol. 210, 808-814, 2016). Here we present a single-particle electron microscopy study of isolated PSII supercomplexes from Norway spruce that revealed binding of a variable amount of LHCII trimers to the PSII core dimer at positions that have never been observed in any other plant species so far. The largest spruce PSII supercomplex, which was found to bind 8 LHCII trimers, is even larger than the current largest known PSII supercomplex from Chlamydomonas reinhardtii. We have also shown that the spruce PSII supercomplexes can form various types of PSII megacomplexes, which were also identified in intact grana membranes. Some of these large PSII supercomplexes and megacomplexes were identified also in Pinus sylvestris, another representative of Pinaceae family. The structural variability and complexity of LHCII organization in Pinaceae seems to be related to the absence of Lhcb6 and Lhcb3 in this family and may be beneficial for the optimization of light-harvesting under varying environmental conditions.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:52:49.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	René Lenobel
SpeciesList	scientific name: Picea abies; NCBI TaxID: 3329;
ModificationList	iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-01 07:36:42	ID requested
⏵ 1	2021-09-09 00:52:49	announced

Kou, ř, il R, Nosek L, Opat, í, kov, á M, Arshad R, Semchonok DA, Chamr, á, d I, Lenobel R, Boekema EJ, Il, í, k P, Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce. Plant J, 104(1):215-225(2020) [pubmed]

submitter keyword: Plant, spruce, membrane, photosystem, complex, LC-MSMS

Roman Kouřil
contact affiliation	Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic
contact email	roman.kouril@upol.cz
lab head
René Lenobel
contact affiliation	Palacky University in Olomouc, Department of protein biochemistry and proteomics
contact email	rene.lenobel@upol.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020138

PRIDE project URI

• PRIDE
  1. PXD020138
     1. Label: PRIDE project
     2. Name: Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce.