PXD020136

PXD020136 is an original dataset announced via ProteomeXchange.

Title	The role of RodAp-conserved cysteine residues in the Aspergillus fumigatus conidial surface organization
Description	Immune inertness of Aspergillus fumigatus conidia, an airborne fungal pathogen, is attributed to its surface rodlet-layer made up of RodAp, a protein belonging to the hydrophobin family, characterized by eight conserved cysteine residues forming four disulfide bonding. Earlier, we showed that the conserved cysteine residue point (ccrp) mutations result in conidia devoid of the rodlet-layer. Here we extended our study in comparing ccrp-mutation with RODA deletion on their mutant conidial surface organization, permeability and immunoreactivity. Western blot using anti-RodAp antibodies indicated the absence of RodAp in the cytoplasm of ccrp-mutant conidia. Upon immunolabelling, ccrp-mutant conidia showed strong positivity for -(1,3)-glucan and weak positivity for -(1,3)-glucan, which was reverse in ∆rodA conidia, and the parental strain conidia were negative; all of their conidial cell wall permeability was similar. Proteomic analyses of the conidial surface exposed proteins of the ccrp-mutants, although lower in number, showed more similarities with the parental strain, but a significant difference with the RODA deletion mutant strain. Further, ccrp-mutant conidia are less immunostimulatory compared to ∆rodA conidia. Together, our data suggest that (i) the conserved cysteine residues are essential for the trafficking of RodAp and the organization of rodlet-layer on the conidial surface, and (ii) point-mutation could be an alternative strategy to study the proteins involved in the organization of fungal cell wall.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:20.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-01 05:53:29	ID requested
1	2021-02-18 07:49:24	announced
⏵ 2	2023-11-14 08:51:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: conidia, surface proteomics, RodA,Aspergillus fumigatus, LC-MS/MS

Axel A. Brakhage
contact affiliation	Department for Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Germany
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020136
     1. Label: PRIDE project
     2. Name: The role of RodAp-conserved cysteine residues in the Aspergillus fumigatus conidial surface organization