PXD020119

PXD020119 is an original dataset announced via ProteomeXchange.

Title	Chemical Proteomics Tracks Virus Entry and Uncovers NCAM1 As Zika Virus Receptor
Description	We present a time-resolved chemical proteomic strategy to track the early-stage entry of ZIKV into host cells. ZIKV was labeled on its surface with a chemical probe, which carries a photocrosslinker to covalently link virus-interacting proteins in living cells on UV exposure at different time points, and a biotin tag for subsequent enrichment and mass spectrometric identification of the receptor or other host proteins critical for virus internalization. We identified Neural Cell Adhesion Molecule (NCAM1) as a potential ZIKV receptor and further validated it through overexpression, knockout, and inhibition of NCAM1 in Vero cells and human glioblastoma cells U-251 MG. Collectively, the strategy can serve as a universal tool to map virus entry pathways and uncover key interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2020-09-11
AnnouncementXML	Submission_2020-09-11_03:30:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Zhang
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-30 00:43:35	ID requested
⏵ 1	2020-09-11 03:30:48	announced

Srivastava M, Zhang Y, Chen J, Sirohi D, Miller A, Zhang Y, Chen Z, Lu H, Xu J, Kuhn RJ, Andy Tao W, Chemical proteomics tracks virus entry and uncovers NCAM1 as Zika virus receptor. Nat Commun, 11(1):3896(2020) [pubmed]

submitter keyword: proteomics;zika;virus

W.Andy Tao
contact affiliation	Purdue University
contact email	taow@purdue.edu
lab head
Ying Zhang
contact affiliation	Fudan university
contact email	ying@fudan.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020119

PRIDE project URI

• PRIDE
  1. PXD020119
     1. Label: PRIDE project
     2. Name: Chemical Proteomics Tracks Virus Entry and Uncovers NCAM1 As Zika Virus Receptor