PXD020112

PXD020112 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals the preliminary physiological differences between the wild and captive spotted seal (Phoca largha) pups
Description	Spotted seals (Phoca largha) is a critically endangered pinniped in China and South Korea. Captive in artificially controlled environment is a conventional method to protect and maintain the population of this species. However, little is known about the physiological differences between the wild and captive P. largha. In order to draw the preliminary protein expression profile in the P. largha, blood from the wild and captive pups were subjected to a label-free comparative proteomic analysis. According to the results, 972 proteins were identified, which performed functions related to various metabolic, immune and cellular processes. Among these identified proteins, the expression level of 51 proteins significantly changed between the wild and captive P. large pups. These differentially expressed proteins were enriched in a wide range of cellular functions, including cytoskeleton, phagocytosis. proteolysis, gene expression regulation and carbohydrate metabolism. The activities of phagocytosis and its related ubiquitin mediated proteolysis were significantly higher in the blood of wild P. largha pups than in captive individuals. In addition, a key protein associated with the differences in the wild and captive P. largha pups, heat shock protein 90-beta, were determined due to the most interactions of it with various differentially expressed proteins. Moreover, the wild P. largha pups could be more nutritionally stressed and have more powerful immune capacity. Our study provides the first data on the preliminary protein composition and gives useful information for the physiological characteristics research in this species.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_03:32:59.820.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zelong Zhao
SpeciesList	scientific name: Phoca largha; NCBI TaxID: 39090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 15:51:12	ID requested
⏵ 1	2021-09-09 03:33:00	announced

Tian J, Du J, Han J, Bao X, Song X, Lu Z, Proteomics reveals the preliminary physiological states of the spotted seal (Phoca largha) pups. Sci Rep, 10(1):18727(2020) [pubmed]

submitter keyword: Spotted seal Phoca largha

Wild

Captive

Proteomics

Phagocytosis

Heat shock protein 90.

Zhichuang Lu
contact affiliation	Dalian Key Laboratory of Conservation Biology for Endangered Marine mammals, Liaoning Ocean and Fisheries Science Research Institute
contact email	luzhichuang@hotmail.com
lab head
Zelong Zhao
contact affiliation	Liaoning Ocean and Fisheries Science Research Institute
contact email	zzl2516@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020112

PRIDE project URI

• PRIDE
  1. PXD020112
     1. Label: PRIDE project
     2. Name: Proteomics reveals the preliminary physiological differences between the wild and captive spotted seal (Phoca largha) pups