PXD020110

PXD020110 is an original dataset announced via ProteomeXchange.

Title	Proteomics Characterization of Mitochondrial-Derived Vesicles Under Oxidative Stress
Description	Mitochondria share attributes of vesicular transport with their bacterial ancestors given their ability to form mitochondrial-derived vesicles (MDVs). MDV formation enhances with stress and has been explored for its potential role in mitochondrial-directed cellular and extracellular signalling. Although the fate of MDVs as a form of mitochondrial quality control and cellular communication has been suggested, the proteomic cargo has remained mostly undefined. In this study, we strategically used an in vitro MDV budding/reconstitution assay, following graded oxidative stress on cardiac mitochondria, to identify and characterize the MDV proteome. Our results confirmed previously identified cardiac MDV markers while also revealing a complete map of the MDV proteome, paving the way to understanding the likely roles for MDV release. MDV cargo was particularly enriched with mitochondrial matrix and inner membrane proteins. Oxidative stress vulnerability of proteins directed the cargo loading, which was enhanced by antimycin A (Ant-A). Among OXPHOS complexes, complexes III and V were found to be Ant-A-sensitive. Proteins from metabolic pathways such as TCA cycle, amino acid, fatty acid and aldehyde metabolism, Fe-S cluster, antioxidant response proteins, autophagy and vesicular processes were also found to be Ant-A sensitive. Intriguingly, proteins containing hyper-reactive cysteine residues (HRCR), which act as redox switches in regulating metabolic pathways, including professional redox enzymes and those that are involved in iron metabolism were found to be loaded in MDVs with Ant-A sensitivity. Lastly, we estimated a possible significant contribution of MDVs to the formation of extracellular vesicles, which can serve as an indicator of mitochondrial stress. In conclusion, using graded oxidative stress, our study gives a layout of MDV proteomics signature, delineates the cargo selectivity and hints at the potential of MDVs and the novel protein cargo identified to serve as vital biomarkers during mitochondrial stress and its related pathologies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:22.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Goutham Vasam
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	L-methionine sulfoxide; monohydroxylated lysine; monohydroxylated leucine; 3-hydroxy-L-phenylalanine; monohydroxylated proline
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 15:50:45	ID requested
1	2021-09-08 11:52:33	announced
⏵ 2	2024-10-22 05:26:26	announced	2024-10-22: Updated project metadata.

10.1096/fj.202002151r;

Vasam G, Nadeau R, Cadete VJJ, Lavall, é, e-Adam M, Menzies KJ, Burelle Y, Proteomics characterization of mitochondrial-derived vesicles under oxidative stress. FASEB J, 35(4):e21278(2021) [pubmed]

submitter keyword: MDV, LC-MSMS, Mitochondria,Rat, Heart, Cardiac mitochondria, Mitochondrial-derived vesicles, Oxidative stress

Yan Burelle
contact affiliation	Professor University Research Chair in Integrative Mitochondrial Biology Interdisciplinary School of Health Sciences, Faculty of Health Sciences University of Ottawa RGN building, 451 Smyth Road, Ottawa, ON K1H 8M5 Canada
contact email	yburell2@uottawa.ca
lab head
Goutham Vasam
contact affiliation	Postdoctoral Fellow
contact email	gvasam@uottawa.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020110

PRIDE project URI

• PRIDE
  1. PXD020110
     1. Label: PRIDE project
     2. Name: Proteomics Characterization of Mitochondrial-Derived Vesicles Under Oxidative Stress