PXD020109

PXD020109 is an original dataset announced via ProteomeXchange.

Title	Differential regulation of extracellular matrix proteins in three recurrent liver metastases of a single patient with colorectal cancer
Description	Colorectal cancer (CRC) patients suffer from the second highest mortality among all cancer entities. In half of all CRC patients, colorectal cancer liver metastases (CRLM) can be observed. Metastatic colorectal cancer is associated with poor overall survival and limited treatment options. Even after successful surgical resection of the primary tumor, metachronous liver metastases occur in one out of eight cases. The only available curative intended treatment is hepatic resection, but metachronous CRLM frequently recur after approximately one year. In this study, we performed a proteome analysis of three recurrent liver metastases of a single CRC patient by mass spectrometry. Despite surgical resection of the primary CRC and adjuvant chemotherapy plus cetuximab treatment, the patient developed three metachronous CRLM which occurred consecutively after 9, 21 and 31 months. We identified a set of 1,132 proteins expressed in the three metachronous CRLM, of which 481 were differentially regulated, including 81 proteins that were associated with the extracellular matrix (ECM). 56 ECM associated proteins were identified as upregulated in the third metastasis, 26 (46%) of which were previously described as negative prognostic markers in CRC, including tenascin C, nidogen 1, fibulin1 and vinculin. These data may reflect an ascending trend of malignancy from the first to the third metachronous colorectal cancer liver metastasis. Additionally, the results indicate different ECM phenotypes for recurrent metachronous metastasis, associated with different grades of malignancy and highlights the importance of individual analysis of molecular features in different, consecutive metastatic events in a single patient.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:06.154.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannah Voß
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 15:50:29	ID requested
1	2022-02-15 13:19:36	announced
⏵ 2	2024-10-22 05:32:08	announced	2024-10-22: Updated project metadata.

10.1007/s10585-020-10058-8;

Vo, ß H, Wurlitzer M, Smit DJ, Ewald F, Alawi M, Spohn M, Indenbirken D, Omidi M, David K, Juhl H, Simon R, Sauter G, Fischer L, Izbicki JR, Molloy MP, Nashan B, Schl, ü, ter H, J, ü, cker M, Differential regulation of extracellular matrix proteins in three recurrent liver metastases of a single patient with colorectal cancer. Clin Exp Metastasis, 37(6):649-656(2020) [pubmed]

submitter keyword: ECM, prognostic factor, colorectal liver metastasis, CRLM, proteomics,colorectal cancer, ECM signatures, metachronous liver metastasis, CRC, extracellular matrix, LC-MS/MS

Prof Dr. Manfred Jücker
contact affiliation	University Medical Center Hamburg-Eppendorf, Institute of Biochemistry and Signal Transduction
contact email	juecker@uke.de
lab head
Hannah Voß
contact affiliation	University Medical Center Hamburg Eppendorf, Institute of Clinical Chemistry and Laboratory Medicine, Group of Mass Spectrometric Proteomics
contact email	ha.voss@uke.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020109
     1. Label: PRIDE project
     2. Name: Differential regulation of extracellular matrix proteins in three recurrent liver metastases of a single patient with colorectal cancer