PXD020105

PXD020105 is an original dataset announced via ProteomeXchange.

Title	APOBEC3C, a perinucleolar protein induced by genotoxins, is recruited to DNA damage sites
Description	The human genome contains 11 APOBEC (Apolipoprotein B mRNA Editing Catalytic Polypeptide-like) cytidine deaminases classified into four families. These proteins function mainly in innate antiviral immunity and can also restrict endogenous retrotransposable element multiplication. The present study focuses on APOBEC3C (A3C), a member of the APOBEC3 sub-family. Some APOBEC3 proteins use their enzymatic activity on genomic DNA, inducing mutations and DNA damage, while other members facilitate DNA repair. Our results show that A3C is highly expressed in cells treated with DNA damaging agents. Its expression is regulated by p53. In addition, we provide the A3C interactome in both normal conditions and in cells exposed to the genotoxin etoposide. Most A3C interacting partners are proteins involved in RNA metabolism and some in DNA repair. Our data also indicate that A3C overexpression is detrimental to mammalian cells and we show that this protein is located around the nucleolus and at laser-damaged DNA sites.
HostingRepository	PRIDE
AnnounceDate	2022-02-07
AnnouncementXML	Submission_2022-02-07_07:48:02.780.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020105
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Patrice Waridel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 07:32:48	ID requested
⏵ 1	2022-02-07 07:48:03	announced

Constantin D, Dubuis G, Conde-Rubio MDC, Widmann C, APOBEC3C, a nucleolar protein induced by genotoxins, is excluded from DNA damage sites. FEBS J, 289(3):808-831(2022) [pubmed]

submitter keyword: APOBEC3C, DNA damage, cancer, genotoxins, human cells

Christian Widmann
contact affiliation	Department of Biomedical Sciences Rue du Bugnon 7 1005 Lausanne Switzerland
contact email	Christian.Widmann@unil.ch
lab head
Patrice Waridel
contact affiliation	University of Lausanne
contact email	patrice.waridel@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD020105

PRIDE project URI

• PRIDE
  1. PXD020105
     1. Label: PRIDE project
     2. Name: APOBEC3C, a perinucleolar protein induced by genotoxins, is recruited to DNA damage sites