PXD020099

PXD020099 is an original dataset announced via ProteomeXchange.

Title	Populus (P. tremula X tremuloides-'T89) Lipidbody proteome
Description	Seed lipid bodies (LB), previously viewed as paradigmatic, possess a restricted proteome and serve carbon and energy storage. Albeit present in minute quantities, LBs are widespread in post-germinative tissues, where they display vigorous movement, mediate transient organellar interactions, and shuttle enzymes, and signaling molecules. Storage as well as motility and signaling are relevant in shoot meristems of woody perennials, which ahead of winter produce buds that like seeds establish dormancy, dehydrate, express OLEOSIN genes and accumulate LBs. Unlike seeds, buds do not completely desiccate and LBs retain potential signaling functions. Here, we mapped bud LB production under dormancy-inducing conditions. We found that over an 8-week period LBs enlarged significantly. Concomitantly, three major OLEOSIN family genes were transiently upregulated at the start of bud formation, while in their wake expression of lipid biosynthesis gene DGAT1a/b and lipase gene SDP1a/b increased. In parallel, LDAPa/b, LDAP2a, and LDAP3a/b were upregulated, especially LDAP1b. All LDAPs were localized to LBs. The conclusion that OLEOSINs were replaced by LDAPs was supported by the finding that PUX10a, PUX10b and CDC498A1, involved in OLESOSIN ubiquitination and degradation, were significantly upregulated. Our proteomic analysis identified 723 putative LB proteins. These included LB-anchored Caleosin and LDIP, peripherally associated LDAP1/3 and OBL1, and numerous proteins that reflect storage, transport, organellar interaction, opportunistic cargo and contaminants. There is a significant overlap with proteins identified earlier in plasmodesmal proteomes. Collectively, the data support a model in which LBs are part of a pre-installed mechanism that serves dormancy release, survival through winter and regrowth.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:23:28.362.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020099
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Manikandan Veerabagu
SpeciesList	scientific name: Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa); NCBI TaxID: 3694;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 05:51:21	ID requested
1	2021-06-29 03:06:04	announced
⏵ 2	2024-10-22 05:23:28	announced	2024-10-22: Updated project metadata.

10.6019/PXD020099;

submitter keyword: Lipid Droplet,Lipidbody, Populus

Prof. Christiaan van der Schoot
contact affiliation	Department of Plant Sciences Tårnbygningen T229 Norwegian University of Life Sciences (NMBU) 1433-Ås, Norway
contact email	chris.vanderschoot@nmbu.no
lab head
Manikandan Veerabagu
contact affiliation	NMBU, IPV
contact email	manikandan.veerabagu@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020099
     1. Label: PRIDE project
     2. Name: Populus (P. tremula X tremuloides-'T89) Lipidbody proteome