PXD020090

PXD020090 is an original dataset announced via ProteomeXchange.

Title	Identification of novel 53BP1 associated proteins
Description	We used homologous recombination based CRISPR Knock-in strategy to insert a SFB tag at C terminal of 53BP1 in 293T cells. We performed tandem affinity purification of endogenous 53BP1 in both soluble and chromatin fraction to uncover known and new 53BP1 binding proteins which might play roles in DNA damage response. We identified and examined FOXK1 for its role in DNA damage response through the interaction with 53BP1 mainly in soluble fraction.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:48.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengfan Tang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 02:19:27	ID requested
1	2021-09-08 23:50:41	announced
⏵ 2	2024-10-22 05:27:52	announced	2024-10-22: Updated project metadata.

Tang M, Feng X, Pei G, Srivastava M, Wang C, Chen Z, Li S, Zhang H, Zhao Z, Li X, Chen J, FOXK1 Participates in DNA Damage Response by Controlling 53BP1 Function. Cell Rep, 32(6):108018(2020) [pubmed]

10.1016/j.celrep.2020.108018;

submitter keyword: 53BP1

Junjie Chen
contact affiliation	Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
contact email	jchen8@mdanderson.org
lab head
Mengfan Tang
contact affiliation	The University of Texas MD Anderson Cancer Center
contact email	mtang@mdanderson.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020090

PRIDE project URI

• PRIDE
  1. PXD020090
     1. Label: PRIDE project
     2. Name: Identification of novel 53BP1 associated proteins