PXD020088

PXD020088 is an original dataset announced via ProteomeXchange.

Title	First identification of ITM2B interactome in the human retina
Description	ITM2B is a type II ubiquitous transmembrane protein which role remains unclear. ITM2B mutations have been associated with different disorders: mutations leading to longer mutant proteins have been reported in two distinct Alzheimer-like autosomal dominant disorders with early-onset progressive dementia, cerebellar ataxia, spasticity and variable degrees of deafness, early onset-cataract and vascular retinal abnormalities. Both disorders share neurological features including severe cerebral amyloid angiopathy, non-neuritic plaques, and fibrillary tangles as in Alzheimer Disease. Our group reported a missense mutation in ITM2B underlying an unusual retinal dystrophy with no dementia. This finding would suggest a specific role of ITM2B in the retina. The identification and characterization of retinal protein partners would bring new insights to the cellular functions of ITM2B in the retina. We performed ITM2B immunoprecipitation followed by quantitative proteomic analysis on normal human retina using two distinct antibodies recognizing different epitopes of the protein. Two pools of ITM2B protein partners were identified for each antibody and compared for functional annotation. Besides common proteins, specific partners involved in translation and mitochondrial homeostasis were identified suggesting a role of ITM2B in these cellular functions. To our knowledge, this is the first report of ITM2B interactome in human retina. Our findings also support a unique and specific role for the newly annotated ITM2B protein isoform.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_01:13:59.047.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 02:18:51	ID requested
⏵ 1	2021-09-09 01:13:59	announced

Wohlschlegel J, Argentini M, Michiels C, Letellier C, Forster V, Condroyer C, He Z, Thuret G, Zeitz C, L, é, ger T, Audo I, First identification of ITM2B interactome in the human retina. Sci Rep, 11(1):17210(2021) [pubmed]

submitter keyword: ITM2B, retina, AP-MS, interactome, human

Isabelle Audo
contact affiliation	Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, France
contact email	isabelle.audo@inserm.fr
lab head
Thibaut LEGER
contact affiliation	EHESP/LERES
contact email	thibaut.leger@ehesp.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020088
     1. Label: PRIDE project
     2. Name: First identification of ITM2B interactome in the human retina