PXD020084

PXD020084 is an original dataset announced via ProteomeXchange.

Title	Cryo-EM structures of dynactin’s shoulder and pointed end show their complex architecture and reveal conserved cargo adaptor binding sites
Description	Dynactin is a 1.2 MDa complex that activates the molecular motor, dynein, for ultra-processive transport along microtubules. In order to do this it forms a tripartite complex with dynein and a coiled-coil adaptor. Dynactin consists of an actin-related filament whose length is defined by its flexible shoulder domain. Despite previous cryo-EM structures, the molecular architecture of the shoulder and pointed end of the filament is still poorly understood due to the lack of high-resolution information in these regions. Here we combine multiple cryo-EM datasets and define precise masking strategies for particle signal subtraction and 3D classification. This overcomes domain flexibility and results in high resolution maps into which we can build the shoulder and pointed end. The unique architecture of the shoulder positions the four identical p50 subunits in different conformations to bind dynactin’s filament, as well as to securely house the p150 subunit. The pointed end map allows us to build the first structure of p62, and reveals the molecular basis for cargo adaptor binding to different sites at the pointed end.
HostingRepository	PRIDE
AnnounceDate	2021-03-19
AnnouncementXML	Submission_2021-03-18_23:06:28.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francis O'Reilly
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 02:18:09	ID requested
⏵ 1	2021-03-18 23:06:29	announced

Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP, Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end. EMBO J, 40(8):e106164(2021) [pubmed]

submitter keyword: Dynactin, crosslinking MS

Juri Rappsilber
contact affiliation	Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany
contact email	rappsilber@tu-berlin.de
lab head
Francis O'Reilly
contact affiliation	TU Berlin
contact email	oreilly@tu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD020084

PRIDE project URI

• PRIDE
  1. PXD020084
     1. Label: PRIDE project
     2. Name: Cryo-EM structures of dynactin’s shoulder and pointed end show their complex architecture and reveal conserved cargo adaptor binding sites