PXD020076

PXD020076 is an original dataset announced via ProteomeXchange.

Title	The spatial network of skeletal proteins in a stony coral
Description	Coral skeletons are materials composed of inorganic aragonitic fibers, proteins, sugars and lipids that are highly organized to form a solid body upon which the animals live. The skeleton contains more than 30 proteins, all of which are encoded in the animal genome and secreted during the biomineralization process. How these proteins are spatially related is unknown. Using a combination of chemical crosslinking and high-resolution tandem mass spectrometry, we identify, for the first time. the spatial interactions of the skeletal proteins within a stony coral. Our subsequent network analysis revealed several coral acid-rich proteins (CARPs) are invariably associated with carbonic anhydrase(s), alpha-collagen, cadherins and other calcium binding proteins. These interactions clearly show that protein-protein interactions in coral skeletons are highly coordinated and are key to understanding the formation and persistence of coral skeletons through time
HostingRepository	PRIDE
AnnounceDate	2021-01-25
AnnouncementXML	Submission_2021-01-24_23:02:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manjula Mummadisetti
SpeciesList	scientific name: Stylophora pistillata; NCBI TaxID: 50429;
ModificationList	crosslinked residues
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 00:08:50	ID requested
⏵ 1	2021-01-24 23:02:47	announced

Dataset with its publication pending

submitter keyword: : Biomineralization, coral reefs, coral acid-rich proteins (CARPs), extracellular matrix (ECM) proteins, mass spectrometry, protein crosslinking, bis(sulfosuccinimidyl)suberate (BS3).

Paul G. Falkowski
contact affiliation	Distinguished Professor, Environmental Biophysics and Molecular Ecology lab, Department of Marine and Coastal Sciences, Rutgers University, New Jersey, U.S.A.
contact email	marinesciencesmanju@gmail.com
lab head
Manjula Mummadisetti
contact affiliation	Rutgers University
contact email	manju@marine.rutgers.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD020076

PRIDE project URI

• PRIDE
  1. PXD020076
     1. Label: PRIDE project
     2. Name: The spatial network of skeletal proteins in a stony coral