PXD020063
PXD020063 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bacterial proteins of Yersinia pestis 201 |
Description | Y. pestis strain 201 cultures grown under Mh (37°C, TMH with no calcium) and Fv (26°C, TMH with 2.5M calcium) conditions were used as the experimental and control groups, respectively. After 8 h of incubation, the bacterial pellets were collected by centfugation, re-suspended in lysis buffer (8 M urea in PBS, pH 7.4) and lysed by sonication in an ice bath. The concentrations of proteins were determined using a Pierce BCA Protein Assay Kit. Tandem mass tag labeling was used to obtain accurate quantitative information to minimize variability between samples. For quantitative analysis, at least two unique peptides were used for the identification and quantification of proteins. |
HostingRepository | iProX |
AnnounceDate | 2020-12-16 |
AnnouncementXML | Submission_2023-03-02_18:37:02.729.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zongmin Du |
SpeciesList | scientific name: Yersinia pestis CO92; NCBI TaxID: 214092; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-06-27 20:27:37 | ID requested | |
1 | 2021-03-14 21:23:58 | announced | |
⏵ 2 | 2023-03-02 18:37:03 | announced | 2023-03-03: Update publication information. |
Publication List
Cao S, Chen Y, Yan Y, Zhu S, Tan Y, Wang T, Song Y, Deng H, Yang R, Du Z, Secretome and Comparative Proteomics of Yersinia pestis Identify Two Novel E3 Ubiquitin Ligases That Contribute to Plague Virulence. Mol Cell Proteomics, 20():100066(2021) [pubmed] |
Keyword List
submitter keyword: proteomics,Yersinia pestis |
Contact List
Ruifu Yang | |
---|---|
contact affiliation | State Key Laboratory of Pathogen and Biosecurity |
contact email | m13801034560@163.com |
lab head | |
Zongmin Du | |
contact affiliation | State Key Laboratory of Pathogen and Biosecurity |
contact email | zmduams@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |