PXD020061
PXD020061 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Parallel reaction monitoring analysis of target proteins |
| Description | Parallel reaction monitoring (PRM) analysis was used to confirm changes in abundance of the target proteins (AceF, SodA, YopD, YopE, GroEL, TerE, and OsmY2),. Equal amounts of proteins from the culture supernatants and cell pellets were separated by SDS-PAGE, and bands corresponding to each of the target proteins were subjected to in-gel digestion. The peptide samples for the target proteins were then analyzed by mass spectrometry, and three peptides from each target protein were selected for the PRM analysis. The selection criterion for the peptides was a sequence length of 6–15 amino acids, while peptides with any modifications or missed cleavage sites were excluded. The peak area of three b-/y-ions from each peptide was used to calculate the peptide ratio, and the ratios of three peptides were used to determine the target protein ratio in different samples. |
| HostingRepository | iProX |
| AnnounceDate | 2020-12-16 |
| AnnouncementXML | Submission_2023-03-02_18:39:13.601.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zongmin Du |
| SpeciesList | scientific name: Yersinia pestis CO92; NCBI TaxID: 214092; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-06-27 20:26:11 | ID requested | |
| 1 | 2021-03-14 21:22:51 | announced | |
| ⏵ 2 | 2023-03-02 18:39:14 | announced | 2023-03-03: Update publication information. |
Publication List
| Cao S, Chen Y, Yan Y, Zhu S, Tan Y, Wang T, Song Y, Deng H, Yang R, Du Z, Secretome and Comparative Proteomics of Yersinia pestis Identify Two Novel E3 Ubiquitin Ligases That Contribute to Plague Virulence. Mol Cell Proteomics, 20():100066(2021) [pubmed] |
Keyword List
| submitter keyword: Yersinia pestistis |
Contact List
| Ruifu Yang | |
|---|---|
| contact affiliation | State Key Laboratory of Pathogen and Biosecurity |
| contact email | m13801034560@163.com |
| lab head | |
| Zongmin Du | |
| contact affiliation | State Key Laboratory of Pathogen and Biosecurity |
| contact email | zmduams@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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