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PXD020061

PXD020061 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleParallel reaction monitoring analysis of target proteins
DescriptionParallel reaction monitoring (PRM) analysis was used to confirm changes in abundance of the target proteins (AceF, SodA, YopD, YopE, GroEL, TerE, and OsmY2),. Equal amounts of proteins from the culture supernatants and cell pellets were separated by SDS-PAGE, and bands corresponding to each of the target proteins were subjected to in-gel digestion. The peptide samples for the target proteins were then analyzed by mass spectrometry, and three peptides from each target protein were selected for the PRM analysis. The selection criterion for the peptides was a sequence length of 6–15 amino acids, while peptides with any modifications or missed cleavage sites were excluded. The peak area of three b-/y-ions from each peptide was used to calculate the peptide ratio, and the ratios of three peptides were used to determine the target protein ratio in different samples.
HostingRepositoryiProX
AnnounceDate2020-12-16
AnnouncementXMLSubmission_2023-03-02_18:39:13.601.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZongmin Du
SpeciesList scientific name: Yersinia pestis CO92; NCBI TaxID: 214092;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-27 20:26:11ID requested
12021-03-14 21:22:51announced
22023-03-02 18:39:14announced2023-03-03: Update publication information.
Publication List
Cao S, Chen Y, Yan Y, Zhu S, Tan Y, Wang T, Song Y, Deng H, Yang R, Du Z, Secretome and Comparative Proteomics of Yersinia pestis Identify Two Novel E3 Ubiquitin Ligases That Contribute to Plague Virulence. Mol Cell Proteomics, 20():100066(2021) [pubmed]
Keyword List
submitter keyword: Yersinia pestistis
Contact List
Ruifu Yang
contact affiliationState Key Laboratory of Pathogen and Biosecurity
contact emailm13801034560@163.com
lab head
Zongmin Du
contact affiliationState Key Laboratory of Pathogen and Biosecurity
contact emailzmduams@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI