PXD020060

PXD020060 is an original dataset announced via ProteomeXchange.

Title	Secreted proteins of Yersinia pestis mutant ΔyscI
Description	Y. pestis strain 201 and mutant ΔyscI cultures grown under Mh (37°C, TMH with no calcium) condition were used as the experimental and control groups, respectively. After 8 h of incubation, the cultures supernatants were collected by centfugation and concentrated using centrifugal filters. The concentrations of proteins were determined using a Pierce BCA Protein Assay Kit. Tandem mass tag labeling was used to obtain accurate quantitative information to minimize variability between samples. For quantitative analysis, at least two unique peptides were used for the identification and quantification of proteins.
HostingRepository	iProX
AnnounceDate	2020-12-16
AnnouncementXML	Submission_2023-03-02_19:42:59.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zongmin Du
SpeciesList	scientific name: Yersinia pestis CO92; NCBI TaxID: 214092;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-27 20:23:22	ID requested
1	2021-03-14 21:49:40	announced
⏵ 2	2023-03-02 19:42:59	announced	2023-03-02: Update publication information.

Cao S, Chen Y, Yan Y, Zhu S, Tan Y, Wang T, Song Y, Deng H, Yang R, Du Z, Secretome and Comparative Proteomics of Yersinia pestis Identify Two Novel E3 Ubiquitin Ligases That Contribute to Plague Virulence. Mol Cell Proteomics, 20():100066(2021) [pubmed]

submitter keyword: Secretome and comparative proteomics of Y. pestis: Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China

Ruifu Yang
contact affiliation	State Key Laboratory of Pathogen and Biosecurity
contact email	m13801034560@163.com
lab head
Zongmin Du
contact affiliation	State Key Laboratory of Pathogen and Biosecurity
contact email	zmduams@163.com
dataset submitter

iProX dataset URI