PXD020055

PXD020055 is an original dataset announced via ProteomeXchange.

Title	Expression profiling of mouse embryonic stem cells by mass spectrometry
Description	Embryonic stem cells from B6 and NOD backgrounds were derived freshly in the presence of 2i. After 3-5 passages on feeders, ES cells were cultured in 2i media without any feeders for several passages. In order to identify differentially expressed genes and proteins, we performed RNA-Seq and mass spectromety analysis respectively. Among the differentially expressed genes, we identified several important players in innate and adaptive immunity. Several of these genes had been linked to onset of type-1 diabetes. Proteomics analysis was able to quantitative differences in protein expression among the B6 and NOD ES cell lines.
HostingRepository	PRIDE
AnnounceDate	2020-08-31
AnnouncementXML	Submission_2020-08-30_22:39:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lars Plate
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-26 18:02:10	ID requested
⏵ 1	2020-08-30 22:39:26	announced

Kirak O, Ke E, Yang KY, Schwarz A, Plate L, Nham A, Abadejos JR, Valencia A, Wiseman RL, Lui KO, Ku M, Premature Activation of Immune Transcription Programs in Autoimmune-Predisposed Mouse Embryonic Stem Cells and Blastocysts. Int J Mol Sci, 21(16):(2020) [pubmed]

submitter keyword: B6, NOD, ESC, LC-MSMS

R. Luke Wiseman
contact affiliation	Scripps Research Institute
contact email	wiseman@scripps.edu
lab head
Lars Plate
contact affiliation	Vanderbilt University
contact email	lars.plate@vanderbilt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD020055

PRIDE project URI

• PRIDE
  1. PXD020055
     1. Label: PRIDE project
     2. Name: Expression profiling of mouse embryonic stem cells by mass spectrometry