PXD020052

PXD020052 is an original dataset announced via ProteomeXchange.

Title	Comprehensive analysis of transcripts and proteins relative abundance during Plasmodium falciparum blood stages
Description	We aimed to evaluate the relevance of mRNA study as a proxy of protein expression in sequestered parasites. To do so, we conducted a proteotranscriptomic analysis using five independent P. falciparum laboratory strains samples. RNA sequencing was performed on circulating ring stage parasites and LC-MS/MS on the corresponding sequestered mature forms. All analyzes were performed within the same development cycle for direct individual correlation. mRNA expression level was assessed at the circulating ring stage and the corresponding protein expression level was measured after 18h-24h of maturation to reach the mature trophozoite stage.
HostingRepository	PRIDE
AnnounceDate	2021-02-10
AnnouncementXML	Submission_2021-02-10_10:57:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Salnot
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-26 14:25:42	ID requested
⏵ 1	2021-02-10 10:57:22	announced

Kamaliddin C, Guillochon E, Salnot V, Rombaut D, Huguet S, Guillonneau F, Houz, é S, Cot M, Deloron P, Argy N, Bertin GI, Infection. J Proteome Res, 20(2):1206-1216(2021) [pubmed]

submitter keyword: Plasmodium falciparum

RNA-seq

FPKM

LC-MS/MS

Label free quantification

Transcriptomes

Proteomes

Transcripts and proteins expression correlation

Gwladys Bertin
contact affiliation	MERIT, IRD, Université de Paris, F-75006 Paris, France
contact email	gwladys.bertin@ird.fr
lab head
Virginie Salnot
contact affiliation	3p5-proteom'ic plateform
contact email	virginie.salnot@parisdescartes.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD020052

PRIDE project URI

• PRIDE
  1. PXD020052
     1. Label: PRIDE project
     2. Name: Comprehensive analysis of transcripts and proteins relative abundance during Plasmodium falciparum blood stages