PXD020049

PXD020049 is an original dataset announced via ProteomeXchange.

Title	Pb stress and ectomycorrhizas: stronger and more effective proteomic responses in poplar roots inoculated with Paxillus involutus isolate characterised by a low root colonization ratio
Description	The commonly observed increased heavy metal tolerance of ectomycorrhized plants is usually linked with the protective role of the fungal hyphae covering colonized plant root tips. However, the molecular effects of ECM-triggered increases in plant mass and the ‘dilution effect’ are unknown. Here, we examined Populus × canescens microcuttings inoculated with the Paxillus involutus isolate, which triggered an increase in poplar growth despite successful colonization of only 1.9% ± 0.8 of root tips. Analyzed plants grown for 6 weeks in agar medium enriched with 0.75 mM Pb(NO3)2 lacked a protective fungal biofilter. In minimally colonized ‘bare’ roots, the proteome response to Pb was similar to that in noninoculated plants (e.g., higher abundances of PM- and V-type H+ 31 ATPases and lower abundance of ribosomal proteins). However, the more intensive activation of molecular processes leading to Pb sequestration or redirection of the root metabolic flux into amino acid and Pb chelate (phenolic and citrate) biosynthesis coexisted with lower Pb uptake compared to that in controls. The molecular Pb response of inoculated roots was more intense and effective than that of noninoculated roots in poplars.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:17.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lukasz Marczak
SpeciesList	scientific name: Populus canescens (Grey poplar) (Populus tremula x Populus alba); NCBI TaxID: 80863;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-26 14:19:02	ID requested
1	2021-09-09 01:20:48	announced
⏵ 2	2024-10-22 05:28:22	announced	2024-10-22: Updated project metadata.

10.3390/ijms22094300;

Szuba A, Marczak Ł, Koz, ł, owski R, Isolate and Characterized by Low Root Colonization Intensity. Int J Mol Sci, 22(9):(2021) [pubmed]

submitter keyword: comparative proteomics, Ectomycorrhiza,Populus canescens, LC-MS/MS

Lukasz Marczak
contact affiliation	Laboratory of Mass Spectrometry, Institute of Bioorganic Chemistry Polish Academy of Sciences
contact email	lukasmar@ibch.poznan.pl
lab head
Lukasz Marczak
contact affiliation	Institute of Bioorganic Chemistry Polish Academy of Sciences
contact email	lukasmar@ibch.poznan.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020049
     1. Label: PRIDE project
     2. Name: Pb stress and ectomycorrhizas: stronger and more effective proteomic responses in poplar roots inoculated with Paxillus involutus isolate characterised by a low root colonization ratio