PXD020020

PXD020020 is an original dataset announced via ProteomeXchange.

Title	Alzheimer disease neocortex LC-MSMS
Description	Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that is pathologically characterized by amyloid β-peptide (Aβ)-containing plaques and the generation of neurofibrillary tangles (NFTs). Although proteome profile changes have been reported in AD brains, it is unclear whether they represent a continuous process from mild to severe changes or whether there is a sequence, with step-by-step involvement of proteins. To address this we analyzed neocortex samples of 19 control cases without signs of neurodegeneration (nonAD), 17 pathologically-diagnosed preclinical AD (p-preAD), and 17 symptomatic AD cases by mass spectrometry after homogenization and separation into soluble, dispersible, membrane-associated, and plaque-associated fractions. Here, we show three classes of proteins exhibiting changed levels during the pathogenesis and progression of AD from the AD pathology-free to the symptomatic stage: five early- and 24 late-responding proteins and 17 gradually changing proteins. The identified proteins and hierarchy point to endocytosis/synaptic vesicle cycle-related processes as key elements in the initiation of AD pathology in the neocortex, while later changes document the resulting neurodegenerative process.
HostingRepository	PRIDE
AnnounceDate	2021-06-21
AnnouncementXML	Submission_2021-06-21_03:12:47.861.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020020
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Xiaohang Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-25 17:03:05	ID requested
⏵ 1	2021-06-21 03:12:48	announced

Li X, Tsolis KC, Koper MJ, Ronisz A, Ospitalieri S, von Arnim CAF, Vandenberghe R, Tousseyn T, Scheuerle A, Economou A, Carpentier S, Otto M, Thal DR, Sequence of proteome profiles in preclinical and symptomatic Alzheimer's disease. Alzheimers Dement, 17(6):946-958(2021) [pubmed]

submitter keyword: human, neocortex, LC-MSMS, Alzheimer

Dietmar Rudolf Thal
contact affiliation	Laboratory for Neuropathology, Department of Imaging and Pathology, KU Leuven (University of Leuven), Leuven, Belgium;Leuven Brain Institute (LBI), KU Leuven (University of Leuven), Leuven, Belgium;Department of Imaging & Pathology, UZ Leuven (University Hospital Leuven), Leuven, Belgium.
contact email	Dietmar.Thal@kuleuven.be
lab head
Xiaohang Li
contact affiliation	Laboratory for Neuropathology, Department of Imaging and Pathology, KU Leuven (University of Leuven), Leuven, Belgium
contact email	xiaohang.li@kuleuven.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD020020

PRIDE project URI

• PRIDE
  1. PXD020020
     1. Label: PRIDE project
     2. Name: Alzheimer disease neocortex LC-MSMS