PXD020008

PXD020008 is an original dataset announced via ProteomeXchange.

Title	CB1 receptor-mediated inhibitory LTD triggers presynaptic remodeling via protein synthesis and ubiquitination
Description	Long-lasting forms of postsynaptic plasticity commonly involve protein synthesis-dependent structural changes of dendritic spines. However, the relationship between protein synthesis and presynaptic structural plasticity remains unclear. Here, we investigated structural changes in cannabinoid-receptor 1 (CB1)-mediated long-term depression of inhibitory transmission (iLTD), a form of presynaptic plasticity that requires protein synthesis and involves a long-lasting reduction in GABA release. We found that CB1-iLTD in acute rat hippocampal slices was associated with protein synthesis-dependent presynaptic structural changes. Using proteomics, we determined that CB1 activation in hippocampal neurons resulted in increased ribosomal proteins and initiation factors, but decreased levels of proteins involved in regulation of the actin cytoskeleton, such as Arp2/3, and presynaptic release. Moreover, while CB1-iLTD increased ubiquitin/proteasome activity, ubiquitination but not proteasomal degradation was critical for structural and functional presynaptic CB1-iLTD. Thus, CB1-iLTD relies on both protein synthesis and ubiquitination to elicit structural changes that underlie long-term reduction of GABA release.
HostingRepository	PRIDE
AnnounceDate	2020-09-23
AnnouncementXML	Submission_2020-09-23_06:11:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020008
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pablo Castillo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-25 01:54:50	ID requested
⏵ 1	2020-09-23 06:11:20	announced

Monday HR, Bourdenx M, Jordan BA, Castillo PE, -receptor-mediated inhibitory LTD triggers presynaptic remodeling via protein synthesis and ubiquitination. Elife, 9():(2020) [pubmed]

submitter keyword: Neurons, cannabinoids, CB1, WIN 55,212-2

Pablo Castillo
contact affiliation	Dominick P. Purpura Department of Neuroscience, Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA
contact email	pablo.castillo@einsteinmed.org
lab head
Pablo Castillo
contact affiliation	Albert Einstein College of Medicine
contact email	pablo.castillo@einsteinmed.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020008

PRIDE project URI

• PRIDE
  1. PXD020008
     1. Label: PRIDE project
     2. Name: CB1 receptor-mediated inhibitory LTD triggers presynaptic remodeling via protein synthesis and ubiquitination