PXD020000

PXD020000 is an original dataset announced via ProteomeXchange.

Title	Downregulation of KRAB zinc finger proteins in 5-fluorouracil resistant colorectal cancer cells
Description	The radio-chemotherapy with 5-fluorouracil (5-FU) is the standard of care treatment for patients with locally advanced rectal cancer (LARC) but it is only effective for a third of them. The proteomic and transcriptomic response of three colorectal cancer (CRC) cell lines to 5-FU and radiation was assessed and correlated with their genetic background. The induction of a 5-FU-resistance in those cell lines negatively affects the levels of transcripts corresponding to Krüppel-associated box (KRAB)-containing zinc finger proteins (ZFPs), the largest family of transcriptional repressors. Among nearly 350 KRAB-ZFPs, almost a quarter are down-regulated after the induction of a 5-FU-resistance including a common one between the three CRC cell lines, ZNF649, whose role is still unknown. This proteomic, transcriptomic and genomic analysis of intrinsic and acquired resistance highlights possible new mechanisms involved in resistance to treatment and therefore potential new therapeutic targets to overcome this resistance.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_09:18:37.849.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francois-Michel Boisvert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-24 07:09:34	ID requested
⏵ 1	2022-05-19 09:18:38	announced

Chauvin A, Bergeron D, Vencic J, L, é, vesque D, Paquette B, Scott MS, Boisvert FM, Downregulation of KRAB zinc finger proteins in 5-fluorouracil resistant colorectal cancer cells. BMC Cancer, 22(1):363(2022) [pubmed]

submitter keyword: colorectal cancer, TMT, protoemics, chemoresistance, 5-FU

François-Michel Boisvert
contact affiliation	Department of Immunology and Cell Biology Université de Sherbrooke 3201 Jean-Mignault, Sherbrooke, Québec, J1E 4K8, Canada
contact email	francois.michel.boisvert@usherbrooke.ca
lab head
Francois-Michel Boisvert
contact affiliation	Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD020000

PRIDE project URI

• PRIDE
  1. PXD020000
     1. Label: PRIDE project
     2. Name: Downregulation of KRAB zinc finger proteins in 5-fluorouracil resistant colorectal cancer cells