PXD019993

PXD019993 is an original dataset announced via ProteomeXchange.

Title	Microtubule-associated serine/threonine-protein kinase-like (MASTL) surfaceome proteomics analysis
Description	Microtubule-associated serine/threonine-protein kinase-like (MASTL; a.k.a. Greatwall (Gwl) kinase) has an established role in the acceleration of cell cycle progression through inhibition of the PP2A-B55 phosphatase activity. Importantly, MASTL has emerged as a putative oncogene. Especially in breast cancer, high MASTL expression levels correlate with increased tumor growth and metastasis in vivo. We reported recently a kinase-independent role for MASTL as a regulator of cell adhesion, contractility and MRTF-A/SRF activity in breast cancer. This study also revealed that depletion of MASTL affects transcription and protein expression of multiple targets. Cell surface proteins are critical for characterization, isolation, and regulation of signaling. In order to reveal if MASTL affects breast cancer cells directly by regulation of surface proteins, we performed SILAC based surfaceome proteomics. Heavy and light isotope labelled endogenously produced surface proteins of MDA-MB-231 cells were purified by biotin enrichment and subjected to proteomics analysis.
HostingRepository	PRIDE
AnnounceDate	2022-06-28
AnnouncementXML	Submission_2022-06-28_02:41:58.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergio Lilla
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-24 06:59:56	ID requested
⏵ 1	2022-06-28 02:41:59	announced

N, ä, rv, ä E, Taskinen ME, Lilla S, Isomursu A, Pietil, ä M, Weltner J, Isola J, Sihto H, Joensuu H, Zanivan S, Norman J, Ivaska J, MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels. iScience, 25(6):104459(2022) [pubmed]

submitter keyword: MASTL, breast cancer, cell contractility, cell adhesion, cell migration, SILAC, surfaceome proteomics

Sara Rossana Zanivan
contact affiliation	CRUK - Beatson Institute for Cancer Research - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom
contact email	s.zanivan@beatson.gla.ac.uk
lab head
Sergio Lilla
contact affiliation	Proteomics
contact email	s.lilla@beatson.gla.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD019993

PRIDE project URI

• PRIDE
  1. PXD019993
     1. Label: PRIDE project
     2. Name: Microtubule-associated serine/threonine-protein kinase-like (MASTL) surfaceome proteomics analysis