PXD019988

PXD019988 is an original dataset announced via ProteomeXchange.

Title	A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress
Description	We have identified a potential selective autophagy receptor protein in Arabidopsis thaliana, C53/AT5G06830/ERP1, that is recruited to ER upon ER-stress activation and induces autophagosome formation. By affinity proteomics IP/MS with ATG8A and E, we identified this new ER-phagy receptor, which is also conserved in metazoans. With biophysical characterization, we revealed its unprecedent binding mode to ATG8 (sAIM). C53 senses proteotoxic stress in the ER lumen by forming a tripartite receptor complex with the ER-associated ufmylation ligase UFL1 and its membrane adaptor DDRGK1. However, its cargo targeted for degradation is still elusive. Initial quantitative proteomics analyses (TMT) of wild type and Atc53 mutant lines revealed that Atc53 mediates degradation of ER resident proteins as well as proteins passaging the ER to the cell wall, apoplast, and lipid droplets.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_03:15:06.629.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	carbamoylated residue; methylthiolated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-24 06:11:07	ID requested
⏵ 1	2021-09-09 03:15:07	announced

Stephani M, Picchianti L, Gajic A, Beveridge R, Skarwan E, Sanchez de Medina Hernandez V, Mohseni A, Clavel M, Zeng Y, Naumann C, Matuszkiewicz M, Turco E, Loefke C, Li B, D, ü, rnberger G, Schutzbier M, Chen HT, Abdrakhmanov A, Savova A, Chia KS, Djamei A, Schaffner I, Abel S, Jiang L, Mechtler K, Ikeda F, Martens S, Clausen T, Dagdas Y, A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress. Elife, 9():(2020) [pubmed]

submitter keyword: Arabidopsis thaliana, whole seedlings, IP/MS, TMT-11plex, Tunicamycin, ER-stress, ER-phagy

Yasin Dagdas
contact affiliation	Gregor Mendel Institute of Molecular Plant Biology GmbH Dr. Bohr-Gasse 3, 1030 Vienna, Austria Phone: +43 1 79044 9850 http://www.gmi.oeaw.ac.at
contact email	yasin.dagdas@gmi.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA - Institute of Molecular Biotechnology
contact email	imre@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019988

PRIDE project URI

• PRIDE
  1. PXD019988
     1. Label: PRIDE project
     2. Name: A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress