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PXD019978

PXD019978 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffect of truncation mutants of the trypanosomatid nuclear lamina protein NUP-1 on the whole cell proteome in T. brucei
DescriptionThe nuclear lamina has multiple functions, including maintaining nuclear structural integrity and differential gene expression. Correct spatial and temporal lamina assembly is essential to meet these and other roles. Recently, it emerged that multiple lamina systems exist that are likely products of independent origins, while all these systems share remarkably analogous functions. Several lamina proteins are known in trypanosomes, two of which, NUP-1 and NUP-2, are essential, coiled-coil proteins with a molecular mass 450 and 250 kDa, respectively. Sequence analysis indicates distinct quaternary structures when compared to the ~60 kDa lamin proteins of multiple lineages, including metazoa. To uncover organisational principles of the trypanosome lamina we generated NUP-1 deletion mutants (N=N-terminal domain; C= C-terminal domain; NC: fusion of the N- and C-terminal domain with entire repeat region deletedd))designed to identify domains of NUP-1 responsible for oligomerisation. We find that both N- and C-termini act as interaction domains and disruption of these interactions impacts additional components of the lamina, the nuclear envelope and nucleoporin TbNup98. By contrast there is remarkably little impact on transcription, crucially including silencing of telomeric variant surface glycoprotein genes. These data indicate that both terminal domains of NUP-1 have roles in assembling the trypanosome lamina and suggest an architecture distinct to the lamin system is based on a ‘hub and spoke’ configuration.
HostingRepositoryPRIDE
AnnounceDate2021-06-03
AnnouncementXMLSubmission_2021-06-03_08:12:45.795.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartin Zoltner
SpeciesList scientific name: Trypanosoma brucei brucei TREU927; NCBI TaxID: 185431;
ModificationListacetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-23 18:15:20ID requested
12021-06-03 08:12:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: lamina, molecular assembly, trypanosoma, nuclear organisation, heterochromatin
Contact List
Mark C. Field
contact affiliationMark C. Field Professor of Cell Biology and Parasitology Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee United Kingdom
contact emailmfield@mac.com
lab head
Martin Zoltner
contact affiliationDivision of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact emailm.zoltner@dundee.ac.uk
dataset submitter
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