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PXD019977 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhactr1/PP1 phosphatase substrates in mouse NIH3T3 fibroblasts
DescriptionIt was previously shown that expression of an activated Phactr1 mutant (Phactr1XXX) that constitutively forms the Phactr1/PP1 phosphatase holoenzyme, induces F-actin rearrangements in NIH3T3 fibroblasts. Expression of the Phactr1 PP1-binding domain (C-terminal) alone is also sufficient to induce such cytoskeletal changes. In contrast, expression of Phactr1XXXC derivative, which lacks the PP1 binding sequences does not result in alteration of cytoskeletal morphology (Wiezlak et al., 2012). These observations suggest that Phactr1/PP1 dephosphorylates target proteins involved in cytoskeletal dynamics. To identify potential Phactr1/PP1 substrates, we used differential SILAC phosphoproteomics in NIH3T3 cells inducibly expressing Phactr1XXX, Phactr1XXXC constructs or vector alone. Over 3000 phosphorylation sites were quantified, among which we determined Phactr1/PP1 target dephosphorylation sites by comparative analysis.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRoman Fedoryshchak
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-23 18:15:01ID requested
12020-10-28 06:32:46announced
Publication List
Fedoryshchak RO, Přechová M, Butler AM, Lee R, O'Reilly N, Flynn HR, Snijders AP, Eder N, Ultanir S, Mouilleron S, Treisman R, Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife, 9():(2020) [pubmed]
Keyword List
submitter keyword: phosphatase, Phactr1, mouse, fibroblasts
Contact List
Richard Treisman
contact affiliationDirector of Research Head, Signalling and Transcription Group The Francis Crick Institute, 1 Midland Road, London, UK
contact emailrichard.treisman@crick.ac.uk
lab head
Roman Fedoryshchak
contact affiliationThe Francis Crick Institute
contact emailroman.fedoryshchak@crick.ac.uk
dataset submitter
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