PXD019977

PXD019977 is an original dataset announced via ProteomeXchange.

Title	Phactr1/PP1 phosphatase substrates in mouse NIH3T3 fibroblasts
Description	It was previously shown that expression of an activated Phactr1 mutant (Phactr1XXX) that constitutively forms the Phactr1/PP1 phosphatase holoenzyme, induces F-actin rearrangements in NIH3T3 fibroblasts. Expression of the Phactr1 PP1-binding domain (C-terminal) alone is also sufficient to induce such cytoskeletal changes. In contrast, expression of Phactr1XXXC derivative, which lacks the PP1 binding sequences does not result in alteration of cytoskeletal morphology (Wiezlak et al., 2012). These observations suggest that Phactr1/PP1 dephosphorylates target proteins involved in cytoskeletal dynamics. To identify potential Phactr1/PP1 substrates, we used differential SILAC phosphoproteomics in NIH3T3 cells inducibly expressing Phactr1XXX, Phactr1XXXC constructs or vector alone. Over 3000 phosphorylation sites were quantified, among which we determined Phactr1/PP1 target dephosphorylation sites by comparative analysis.
HostingRepository	PRIDE
AnnounceDate	2020-10-28
AnnouncementXML	Submission_2020-10-28_06:32:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fedoryshchak
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-23 18:15:01	ID requested
⏵ 1	2020-10-28 06:32:46	announced

Fedoryshchak RO, P, ř, echov, á M, Butler AM, Lee R, O'Reilly N, Flynn HR, Snijders AP, Eder N, Ultanir S, Mouilleron S, Treisman R, Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife, 9():(2020) [pubmed]

submitter keyword: phosphatase, Phactr1, mouse, fibroblasts

Richard Treisman
contact affiliation	Director of Research Head, Signalling and Transcription Group The Francis Crick Institute, 1 Midland Road, London, UK
contact email	richard.treisman@crick.ac.uk
lab head
Roman Fedoryshchak
contact affiliation	The Francis Crick Institute
contact email	roman.fedoryshchak@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019977
     1. Label: PRIDE project
     2. Name: Phactr1/PP1 phosphatase substrates in mouse NIH3T3 fibroblasts