⮝ Full datasets listing

PXD019974

PXD019974 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons
DescriptionTendon is a highly organized, dense connective tissue that has been demonstrated to have very little turnover. In spite of the low turnover, tendon can grow in response to loading, which may take place primarily at the periphery. Tendon injuries and recurrence of injuries are common in both human and animal in sports. It is unclear why some areas of the tendon are more susceptible to such injury and whether this is due to intrinsic regional differences in extracellular matrix (ECM) production or tissue turnover. This study aimed to compare populations of tenocytes derived from the tendon core and periphery. Tenocytes were isolated from equine superficial digital flexor tendons (SDFT), and the proliferation capacity was determined. ECM production was characterized by immuno- and histological staining and by liquid chromatography-mass spectrometry-based proteomics. Core and periphery SDFT cultures exhibited comparable proliferation rates and had very similar proteome profiles, but showed biological variation in collagen type I deposition. In conclusion, the intrinsic properties of tenocytes from different regions of the tendon are very similar and other factors in the tissue may contribute to how specific areas respond to loading or injury.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:11:17.585.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterErwin Schoof
SpeciesList scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationListcarbamoylated residue; monohydroxylated residue; acetylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-23 18:13:55ID requested
12020-08-23 23:37:45announced
22024-10-22 05:11:19announced2024-10-22: Updated project metadata.
Publication List
Zhang C, Svensson RB, Montagna C, Carstensen H, Buhl R, Schoof EM, Kjaer M, Magnusson SP, Yeung CC, Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons. J Proteome Res, 19(10):4137-4144(2020) [pubmed]
10.1021/acs.jproteome.0c00591;
Keyword List
submitter keyword: shotgun proteomics, tendon,LC-MSMS
Contact List
Chloe Yeung
contact affiliationInstitute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark
contact emailchloe.yeung@gmail.com
lab head
Erwin Schoof
contact affiliationTechnical University of Denmark
contact emailerws@dtu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019974
PRIDE project URI
Repository Record List
[ + ]