PXD019974 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons |
Description | Tendon is a highly organized, dense connective tissue that has been demonstrated to have very little turnover. In spite of the low turnover, tendon can grow in response to loading, which may take place primarily at the periphery. Tendon injuries and recurrence of injuries are common in both human and animal in sports. It is unclear why some areas of the tendon are more susceptible to such injury and whether this is due to intrinsic regional differences in extracellular matrix (ECM) production or tissue turnover. This study aimed to compare populations of tenocytes derived from the tendon core and periphery. Tenocytes were isolated from equine superficial digital flexor tendons (SDFT), and the proliferation capacity was determined. ECM production was characterized by immuno- and histological staining and by liquid chromatography-mass spectrometry-based proteomics. Core and periphery SDFT cultures exhibited comparable proliferation rates and had very similar proteome profiles, but showed biological variation in collagen type I deposition. In conclusion, the intrinsic properties of tenocytes from different regions of the tendon are very similar and other factors in the tissue may contribute to how specific areas respond to loading or injury. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:11:17.585.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Erwin Schoof |
SpeciesList | scientific name: Equus caballus (Horse); NCBI TaxID: 9796; |
ModificationList | carbamoylated residue; monohydroxylated residue; acetylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-23 18:13:55 | ID requested | |
1 | 2020-08-23 23:37:45 | announced | |
⏵ 2 | 2024-10-22 05:11:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
Zhang C, Svensson RB, Montagna C, Carstensen H, Buhl R, Schoof EM, Kjaer M, Magnusson SP, Yeung CC, Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons. J Proteome Res, 19(10):4137-4144(2020) [pubmed] |
10.1021/acs.jproteome.0c00591; |
Keyword List
submitter keyword: shotgun proteomics, tendon,LC-MSMS |
Contact List
Chloe Yeung |
contact affiliation | Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark |
contact email | chloe.yeung@gmail.com |
lab head | |
Erwin Schoof |
contact affiliation | Technical University of Denmark |
contact email | erws@dtu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019974
- Label: PRIDE project
- Name: A Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons