PXD019974

PXD019974 is an original dataset announced via ProteomeXchange.

Title	A Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons
Description	Tendon is a highly organized, dense connective tissue that has been demonstrated to have very little turnover. In spite of the low turnover, tendon can grow in response to loading, which may take place primarily at the periphery. Tendon injuries and recurrence of injuries are common in both human and animal in sports. It is unclear why some areas of the tendon are more susceptible to such injury and whether this is due to intrinsic regional differences in extracellular matrix (ECM) production or tissue turnover. This study aimed to compare populations of tenocytes derived from the tendon core and periphery. Tenocytes were isolated from equine superficial digital flexor tendons (SDFT), and the proliferation capacity was determined. ECM production was characterized by immuno- and histological staining and by liquid chromatography-mass spectrometry-based proteomics. Core and periphery SDFT cultures exhibited comparable proliferation rates and had very similar proteome profiles, but showed biological variation in collagen type I deposition. In conclusion, the intrinsic properties of tenocytes from different regions of the tendon are very similar and other factors in the tissue may contribute to how specific areas respond to loading or injury.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:17.585.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erwin Schoof
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-23 18:13:55	ID requested
1	2020-08-23 23:37:45	announced
⏵ 2	2024-10-22 05:11:19	announced	2024-10-22: Updated project metadata.

Zhang C, Svensson RB, Montagna C, Carstensen H, Buhl R, Schoof EM, Kjaer M, Magnusson SP, Yeung CC, Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons. J Proteome Res, 19(10):4137-4144(2020) [pubmed]

10.1021/acs.jproteome.0c00591;

submitter keyword: shotgun proteomics, tendon,LC-MSMS

Chloe Yeung
contact affiliation	Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark
contact email	chloe.yeung@gmail.com
lab head
Erwin Schoof
contact affiliation	Technical University of Denmark
contact email	erws@dtu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019974

PRIDE project URI

• PRIDE
  1. PXD019974
     1. Label: PRIDE project
     2. Name: A Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons