PXD019972

PXD019972 is an original dataset announced via ProteomeXchange.

Title	Identification of candidate circulating glucocorticoid response biomarkers in humans using a proteomic approach
Description	Context Glucocorticoids used in pharmacological doses for the treatment of a variety of medical conditions, and endogenous glucocorticoid excess – Cushing’s syndrome, may result in several adverse effects, but currently there is no clinically useful biomarker of glucocorticoid activity. Objective To identify glucocorticoid-responsive proteins potentially measurable in human serum. Setting and Design A three-phase protein biomarker discovery strategy was used. Proteomic biomarker discovery and qualification was conducted on the secretome of ex vivo-stimulated peripheral blood mononuclear cells (PBMC) isolated from 6 volunteers, incubated ± dexamethasone 100 ng/mL for 4h and 24h. Untargeted proteomics with label-free quantification (LFQ) was conducted to discover candidate proteins which were quantified using targeted proteomics by a custom multiple reaction monitoring mass spectrometry (MRM-MS) assay. Five proteins were selected for serum measurement by immunoassay in 20 healthy volunteers, with blood drawn at baseline and 12h after 4 mg oral dexamethasone.
HostingRepository	PanoramaPublic
AnnounceDate	2021-03-15
AnnouncementXML	Submission_2021-03-15_07:05:40.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ahmed Mohamed
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	LCMS-8050

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-23 16:11:57	ID requested
⏵ 1	2021-03-15 07:05:41	announced

Inder WJ, Mohamed A, Keshvari S, Barclay JL, Ruelcke JE, Stoll T, Nolan BJ, Cesana-Nigro N, Hill MM, Ex vivo glucocorticoid-induced secreted proteome approach for discovery of glucocorticoid-responsive proteins in human serum. Proteomics Clin Appl, 15(2-3):e2000078(2021) [pubmed]

submitter keyword: Proteomics, PBMC, glucocorticoid, dexamethasone, biomarker

Michelle Hill
contact affiliation	QIMR Berghofer Medical Research Institute
contact email	michelle.hill@qimrberghofer.edu.au
lab head
Ahmed Mohamed
contact affiliation	QIMR Berghofer Medical Research Institute
contact email	ahmed.mohamed@qimrberghofer.edu.au
dataset submitter

Panorama Public dataset URI