PXD019967

PXD019967 is an original dataset announced via ProteomeXchange.

Title	Proteomic analyses reveal that immune integrins are major targets for proteolysis by Membrane-Associated Ring-CH (MARCH) proteins MARCH2, 3, 4 and 9.
Description	MARCH proteins are membrane-associated Ring-CH E3 ubiquitin ligases that dampen immune responses by down-regulating cell surface expression of major histocompatibility complexes I and II as well as immune co-stimulatory receptors. We recently showed that MARCH2,3,4 and 9 also down-regulate cell surface expression of the inflammatory cytokine receptor for interleukin-6 (IL6Ra). We used over-expression of these MARCH proteins in the M1 myeloid leukemia cell line and cell surface proteomic analyses to globally analyse other potential targets of these proteins. A large range of overlapping and a few unique cell surface proteins were identified most of which were down-regulated by MARCH expression. Prominent amongst these were several integrin complexes associated with immune cell homing, adhesion and migration. Integrin 41 (VLA4 or VCAM-1 receptor) was down-regulated only by MARCH2 and we showed that in MARCH2 knockout mice Integrin 4 was up-regulated specifically in mature B-lymphocytes and this was accompanied by decreased numbers of B-cells in the spleen.
HostingRepository	PRIDE
AnnounceDate	2021-05-07
AnnouncementXML	Submission_2021-05-06_23:13:46.594.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jarrod Sandow
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-22 23:27:31	ID requested
⏵ 1	2021-05-06 23:13:47	announced

Sandow JJ, Webb AI, Stockwell D, Kershaw NJ, Tan C, Ishido S, Alexander WS, Hilton DJ, Babon JJ, Nicola NA, Proteomic analyses reveal that immune integrins are major targets for regulation by Membrane-Associated Ring-CH (MARCH) proteins MARCH2, 3, 4 and 9. Proteomics, 21(11-12):e2000244(2021) [pubmed]

submitter keyword: Cell Surface, Integrins, Immune cells,

Nicos A Nicola
contact affiliation	The Walter and Eliza Hall Institute of Medical Research
contact email	nicola@wehi.edu.au
lab head
Jarrod Sandow
contact affiliation	WEHI
contact email	sandow@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD019967

PRIDE project URI

• PRIDE
  1. PXD019967
     1. Label: PRIDE project
     2. Name: Proteomic analyses reveal that immune integrins are major targets for proteolysis by Membrane-Associated Ring-CH (MARCH) proteins MARCH2, 3, 4 and 9.