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PXD019952

PXD019952 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnravelling metabolic cross-feeding in a yeast-bacteria community using 13C proteomics
DescriptionCross-feeding is fundamental to the diversity and function of microbial communities. However, identification of cross-fed metabolites is often challenging due to the universality of metabolic and biosynthetic intermediates. Here, we use 13C isotope tracing in peptides to elucidate cross-fed metabolites in cocultures of Saccharomyces cerevisiae and Lactococcus lactis. The community was grown on lactose as the main carbon source with either glucose or galactose fraction of the molecule labelled with 13C. Data analysis allowing for the possible mass-shifts yielded hundreds of peptides for which we could assign both species identity and labelling degree. The labelling pattern showed that the yeast utilized galactose and, to a lesser extent, lactic acid shared by L. lactis as carbon sources. While the yeast provided essential amino acids to the bacterium as expected, the data also uncovered a complex pattern of amino acid exchange. The identity of the cross-fed metabolites was further supported by metabolite labelling in the co-culture supernatant, and by diminished fitness of a galactose-negative yeast mutant in the community. Together, our results demonstrate the utility of 13C-based proteomics for uncovering microbial interactions.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:07.625.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD019952
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristoniki Maga-Nteve
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-22 07:53:49ID requested
12023-02-07 05:59:25announced
22023-11-14 08:51:08announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD019952;
Keyword List
submitter keyword: protein stable-isotope labeling (protein-SIP), cross-feeding, synthetic community,: microbial interaction
Contact List
Kiran Raosaheb Patil
contact affiliationGroup Leader, Structural and Computational Biology Unit, European Molecular Biology Laboratory Director of Research, MRC Toxicology Unit, University of Cambridge
contact emailkiran.patil@embl.de
lab head
Christoniki Maga-Nteve
contact affiliationEMBL
contact emailchristoniki.maga@embl.de
dataset submitter
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Dataset FTP location
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