PXD019930

PXD019930 is an original dataset announced via ProteomeXchange.

Title	Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases
Description	Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo-β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β-D-glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:26:01.757.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bogdan Florea
SpeciesList	scientific name: Aspergillus niger NRRL3; NCBI TaxID: 1451139;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-22 03:08:27	ID requested
⏵ 1	2021-09-09 00:26:02	announced

de Boer C, McGregor NGS, Peterse E, Schr, ö, der SP, Florea BI, Jiang J, Reijngoud J, Ram AFJ, van Wezel GP, van der Marel GA, Cod, é, e JDC, Overkleeft HS, Davies GJ, Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases. RSC Chem Biol, 1(3):148-155(2020) [pubmed]

submitter keyword: cyclophellitol, activity-based probe, ABPP, cellulases

Bogdan I Florea
contact affiliation	Bio-organic chemistry, LIC, Leiden University, Einsteinweg 55, 2333CC, Leiden, The Netherlands
contact email	b.florea@chem.leidenuniv.nl
lab head
Bogdan Florea
contact affiliation	Leiden Institute for Chemistry
contact email	b.florea@chem.leidenuniv.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019930

PRIDE project URI

• PRIDE
  1. PXD019930
     1. Label: PRIDE project
     2. Name: Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases