PXD019922

PXD019922 is an original dataset announced via ProteomeXchange.

Title	Mesoplasma florum proteome analysis by 2D LC-MS/MS
Description	The near-minimal bacterium Mesoplasma florum constitutes an interesting model for synthetic genomics and systems biology studies due to its small genome, fast growth rate, and lack of pathogenic potential. However, some fundamental aspects of its biology remain largely unexplored. Here, we report a broad yet remarkably detailed characterization of M. florum by combining a wide variety of experimental approaches. More specifically, we investigated several physical and physiological aspects of this bacterium, and performed the first genome-wide analysis of its transcriptome and proteome using RNA sequencing techniques and two-dimensional liquid chromatography-tandem mass spectrometry. These analyses notably revealed the M. florum consensus promoter, a first experimental cartography of transcription units, as well as the transcription and expression levels of all annotated genes. We also converted gene transcription and expression levels into absolute molecular abundances using biomass quantification results, generating an unprecedented view of the M. florum cellular composition and functions. These characterization efforts will provide an experimental foundation for the development of a genome-scale metabolic model for M. florum and will guide future genome engineering endeavours in this simple organism.
HostingRepository	PRIDE
AnnounceDate	2020-11-16
AnnouncementXML	Submission_2020-11-16_07:56:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019922
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sébastien Rodrigue
SpeciesList	scientific name: Mesoplasma florum L1; NCBI TaxID: 265311;
ModificationList	phosphorylated residue; acetylated residue; formylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-21 23:38:08	ID requested
⏵ 1	2020-11-16 07:56:30	announced

Dataset with its publication pending

submitter keyword: Mesoplasma florum, Mollicutes, 2D LC-MS/MS

Sébastien Rodrigue
contact affiliation	Université de Sherbrooke, Département de biologie 2500 boul. de l'Université, Sherbrooke (Québec), Canada J1K 2R1
contact email	Sebastien.Rodrigue@usherbrooke.ca
lab head
Sébastien Rodrigue
contact affiliation	Université de Sherbrooke
contact email	Sebastien.rodrigue@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019922

PRIDE project URI

• PRIDE
  1. PXD019922
     1. Label: PRIDE project
     2. Name: Mesoplasma florum proteome analysis by 2D LC-MS/MS