PXD019917

PXD019917 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins exhibiting TgDegP2-responsive thermal stability
Description	To broadly explore artemisinin susceptibility in apicomplexan parasites, we used genome-scale CRISPR screens recently developed for Toxoplasma gondii to discover sensitizing and desensitizing mutations. From these screens, we identified the mitochondrial protease DegP2, which appeared gDegP2-responsive thermal stability in a pathway leading to decreased DHA susceptibility. To identify proteins exhibiting DegP2-responsive thermal stability
HostingRepository	PRIDE
AnnounceDate	2020-08-28
AnnouncementXML	Submission_2020-09-27_23:34:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alice Herneisen
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; phosphorylated residue; isotope labeled residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-21 17:03:05	ID requested
1	2020-08-28 07:16:58	announced
⏵ 2	2020-09-27 23:34:43	announced	2020-09-28: Updated publication reference for PubMed record(s): 32968076.

Harding CR, Sidik SM, Petrova B, Gn, ä, dig NF, Okombo J, Herneisen AL, Ward KE, Markus BM, Boydston EA, Fidock DA, Lourido S, Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility. Nat Commun, 11(1):4813(2020) [pubmed]

submitter keyword: Toxoplasma gondii, thermal proteome profiling, CETSA, artemisinin susceptibility

Sebastian Lourido
contact affiliation	Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Biology, Cambridge, MA, USA
contact email	lourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact email	alice@wi.mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019917

PRIDE project URI

• PRIDE
  1. PXD019917
     1. Label: PRIDE project
     2. Name: Identification of proteins exhibiting TgDegP2-responsive thermal stability