PXD019908

PXD019908 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of rat primary myocyte and neuron-myocyte co-cultures
Description	Alterations in autonomic function are known to occur in cardiac conditions including sudden cardiac death. Cardiac stimulation via sympathetic neurons can potentially trigger arrhythmias. Dissecting direct neural-cardiac interactions at the cellular level is technically challenging and understudied due to the lack of experimental model systems and methodologies. Here we demonstrate the utility of optical interrogation of sympathetic neurons and their effects on macroscopic cardiomyocyte network dynamics to address research targets such as the effects of adrenergic stimulation via the release of neurotransmitters, the effect of neuronal numbers on cardiac wave behaviour and the applicability of optogenetics in mechanistic in vitro studies. We present novel methodologies to study neuron-cardiomyocyte interactions involving optogenetic selective probing and all-optical electrophysiology to measure electrical activity in an automated fashion, illustrating the power and high-throughput capability of such interrogations. We present new findings on how neurons impact cardiac macroscopic wave properties, the links between neuron density and cardiac firing rates as well as the challenges and benefits of macroscopic co-cultures as experimental model systems.
HostingRepository	PRIDE
AnnounceDate	2020-07-21
AnnouncementXML	Submission_2020-07-21_07:13:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019908
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Holger Kramer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-19 08:54:23	ID requested
⏵ 1	2020-07-21 07:13:16	announced

Burton RB, Tomek J, Ambrosi CM, Larsen HE, Sharkey AR, Capel RA, Corbett AD, Bilton S, Klimas A, Stephens G, Cremer M, Bose SJ, Li D, Gallone G, Herring N, Mann EO, Kumar A, Kramer H, Entcheva E, Paterson DJ, Bub G, Optical Interrogation of Sympathetic Neuronal Effects on Macroscopic Cardiomyocyte Network Dynamics. iScience, 23(7):101334(2020) [pubmed]

submitter keyword: cardiomyocyte network dynamics

cardiac arrhythmias

myocyte

co-culture

Holger B R Kramer
contact affiliation	Proteomics & Metabolomics Facility MRC London Institute of Medical Sciences, Du Cane Road, London W12 0NN, United Kingdom
contact email	h.kramer@lms.mrc.ac.uk
lab head
Holger Kramer
contact affiliation	MRC London Institute of Medical Sciences
contact email	h.kramer@lms.mrc.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019908

PRIDE project URI

• PRIDE
  1. PXD019908
     1. Label: PRIDE project
     2. Name: Proteomics analysis of rat primary myocyte and neuron-myocyte co-cultures