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PXD019906

PXD019906 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteins associated with Cdc48A identified by LC-MS\MS data
DescriptionHis6-Cdc48a and associated proteins purified from H. volcanii. A) SDS- PAGE of proteins purified by Ni2+ -chromatography. H. volcanii carrying empty vector control (H1209-pJAM202c, lane 1) and ectopically expressing His6-Cdc48a (H1209- pJAM1409, lane 2). Red bars indicate regions of gel excised. B) Proteins identified in gel slices by LC-MS/MS analysis. Criteria for protein inclusion: FDR < 0.01%, normalized total spectra minus control > 50, protein threshold > 99% and 2 peptide minimum. Based on spectral counting, Cdc48a and DUF111 were most abundant in regions (a) and (b), respectively, as indicated. DHS1, deoxyhypusine synthase. DUF111, Cdc48a gene neighbor of unknown function. TnaA, tryptophanase; Rps3, ribosomal protein S3; n.d., not detected.
HostingRepositoryPRIDE
AnnounceDate2023-06-22
AnnouncementXMLSubmission_2023-06-22_14:33:19.932.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD019906
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSwathiDantuluri
SpeciesList scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-19 06:28:18ID requested
12023-06-22 14:33:20announced
Publication List
10.6019/PXD019906;
Keyword List
submitter keyword: Cdc48A, HVO_2380
Contact List
JulieMaupin-Furlow
contact affiliationProfessor,Microbiology and Cell Science Department, University Of Florida, USA.
contact emailjmaupin@ufl.edu
lab head
SwathiDantuluri
contact affiliationMemorial Sloan Kettering Cancer CE
contact emaildantulus@mskcc.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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