PXD019897

PXD019897 is an original dataset announced via ProteomeXchange.

Title	Cdc48A pulldown from an amino lonk column baited with SAMP1
Description	H. volcanii Cdc48a, RecJ3/4 and RNase J associate with Ubl-coated beads. A) Strategy to isolate H. volcanii proteins that bind agarose beads charged with monomeric Ubl (vs. BSA) as bait. Cell lysate was from triplicate cultures of H. volcanii NH02-pJAM957. B) Left, Non-reducing SDS-PAGE of H. volcanii proteins that bound BSA- (control, lane 1) vs. Ubl- (lane 2) decorated beads in the presence of ATP. Gels were stained with SYPRO Ruby. Red bar, HMW(250kDa) and LMW(50kDa) (high and low molecular weight) regions of gel excised from lanes 1 and 2 for LC- MS/MS analysis. Right, Proteins identified at > 99.9% probability and < 0.1 % false discovery rate (FDR) in the Ubl (not BSA) samples. Similar trend was observed in independent experiment. ATP was required for this association. Theor. Mr, theoretical molecular mass based on genome sequence. Normalized total spectra of sample minus control is indicated.
HostingRepository	PRIDE
AnnounceDate	2023-06-22
AnnouncementXML	Submission_2023-06-22_14:32:13.916.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019897
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SwathiDantuluri
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-19 03:05:52	ID requested
⏵ 1	2023-06-22 14:32:14	announced

10.6019/PXD019897;

submitter keyword: Cdc48A, SAMP1, HVO_2380

Julie MaupinFurlow
contact affiliation	Professor, Microbiology and Cell Science at University Of Florida.
contact email	jmaupin@ufl.edu
lab head
SwathiDantuluri
contact affiliation	Memorial Sloan Kettering Cancer CE
contact email	dantulus@mskcc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD019897

PRIDE project URI

• PRIDE
  1. PXD019897
     1. Label: PRIDE project
     2. Name: Cdc48A pulldown from an amino lonk column baited with SAMP1