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PXD019882

PXD019882 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhactr1/PP1 phosphatase substrates in mouse primary neurons
DescriptionNeurons express Phactr1 at high level (Allen et al., 2004), and Phactr1 mutations are associated with morphological and functional developmental defects in cortical neurons (Hamada et al., 2018). To identify targets of the Phactr1/PP1 phosphatase holoenzyme, we cultured hippocampal and cortical neurons from wildtype and Phactr1-null animals, treated them either with Latrunculin B or Cytochalasin D to inhibit or activate formation of the Phactr1/PP1 complex (Wiezlak et al., 2012), and analysed phosphorylation profiles using TMT phosphoproteomics. Among over 9000 phosphorylation sites quantified, we found numerous phosphorylation sites that differed significantly in their response to these stimuli in wildtype as opposed to Phactr1-null neurons.
HostingRepositoryPRIDE
AnnounceDate2020-10-28
AnnouncementXMLSubmission_2020-10-28_06:30:00.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRoman Fedoryshchak
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-18 22:48:22ID requested
12020-10-28 06:30:01announced
Publication List
Fedoryshchak RO, Přechová M, Butler AM, Lee R, O'Reilly N, Flynn HR, Snijders AP, Eder N, Ultanir S, Mouilleron S, Treisman R, Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife, 9():(2020) [pubmed]
Keyword List
submitter keyword: phosphatase, Phactr1, mouse, neurons
Contact List
Richard Treisman
contact affiliationDirector of Research Head, Signalling and Transcription Group The Francis Crick Institute 1 Midland Road, London NW11AT, UK
contact emailrichard.treisman@crick.ac.uk
lab head
Roman Fedoryshchak
contact affiliationThe Francis Crick Institute
contact emailroman.fedoryshchak@crick.ac.uk
dataset submitter
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Dataset FTP location
PRIDE project URI
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