PXD019882

PXD019882 is an original dataset announced via ProteomeXchange.

Title	Phactr1/PP1 phosphatase substrates in mouse primary neurons
Description	Neurons express Phactr1 at high level (Allen et al., 2004), and Phactr1 mutations are associated with morphological and functional developmental defects in cortical neurons (Hamada et al., 2018). To identify targets of the Phactr1/PP1 phosphatase holoenzyme, we cultured hippocampal and cortical neurons from wildtype and Phactr1-null animals, treated them either with Latrunculin B or Cytochalasin D to inhibit or activate formation of the Phactr1/PP1 complex (Wiezlak et al., 2012), and analysed phosphorylation profiles using TMT phosphoproteomics. Among over 9000 phosphorylation sites quantified, we found numerous phosphorylation sites that differed significantly in their response to these stimuli in wildtype as opposed to Phactr1-null neurons.
HostingRepository	PRIDE
AnnounceDate	2020-10-28
AnnouncementXML	Submission_2020-10-28_06:30:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fedoryshchak
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-18 22:48:22	ID requested
⏵ 1	2020-10-28 06:30:01	announced

Fedoryshchak RO, P, ř, echov, á M, Butler AM, Lee R, O'Reilly N, Flynn HR, Snijders AP, Eder N, Ultanir S, Mouilleron S, Treisman R, Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife, 9():(2020) [pubmed]

submitter keyword: phosphatase, Phactr1, mouse, neurons

Richard Treisman
contact affiliation	Director of Research Head, Signalling and Transcription Group The Francis Crick Institute 1 Midland Road, London NW11AT, UK
contact email	richard.treisman@crick.ac.uk
lab head
Roman Fedoryshchak
contact affiliation	The Francis Crick Institute
contact email	roman.fedoryshchak@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019882
     1. Label: PRIDE project
     2. Name: Phactr1/PP1 phosphatase substrates in mouse primary neurons