PXD019881 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isobaric matching between runs and novel PSM-level normalization in MaxQuant strongly improve reporter ion-based quantification - Peli1 knock-out mice dataset |
Description | Isobaric labeling has the promise of combining high sample multiplexing with precise quantification. However, normalization issues and the missing value problem of complete n-plexes hamper quantification across more than one n-plex. Here we introduce two novel algorithms implemented in MaxQuant that substantially improve the data analysis with multiple n-plexes. First, isobaric matching between runs (IMBR) makes use of the three-dimensional MS1 features to transfer identifications from identified to unidentified MS/MS spectra between LC-MS runs in order to utilize reporter ion intensities in unidentified spectra for quantification. On typical datasets, we observe a significant gain in quantifiable n-plexesMS/MS spectra that can be used for quantification. Second, we introduce a novel PSM-level normalization, applicable to data with and without common reference channel. It is a weighted median-based method, in which the weights reflect the number of ions that were used for fragmentation. On a typical dataset, we observe complete removal of batch effects and dominance of the biological sample grouping after normalization. This dataset is one of the datasets used for the study. It is TMT 10-plex with a reference channel. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:07:36.421.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sung-Huan Yu |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-18 22:44:32 | ID requested | |
1 | 2020-06-21 22:20:57 | announced | |
2 | 2020-09-07 22:55:46 | announced | 2020-09-08: Updated publication reference for PubMed record(s): 32892627. |
⏵ 3 | 2024-10-22 05:07:41 | announced | 2024-10-22: Updated project metadata. |
Publication List
Yu SH, Kyriakidou P, Cox J, Isobaric Matching between Runs and Novel PSM-Level Normalization in MaxQuant Strongly Improve Reporter Ion-Based Quantification. J Proteome Res, 19(10):3945-3954(2020) [pubmed] |
10.1021/acs.jproteome.0c00209; |
Keyword List
submitter keyword: match between runs, normalization, tandem mass tag,isobaric labeling, multiplexed quantification |
Contact List
Jürgen Cox |
contact affiliation | Max Planck Institute of Biochemistry |
contact email | cox@biochem.mpg.de |
lab head | |
Sung-Huan Yu |
contact affiliation | Max Planck Institute of BIochemistry |
contact email | shyu@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019881
- Label: PRIDE project
- Name: Isobaric matching between runs and novel PSM-level normalization in MaxQuant strongly improve reporter ion-based quantification - Peli1 knock-out mice dataset