PXD019874
PXD019874 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CD20 is gatekeeper of human B cell identity and activation state |
| Description | B cell identity and function is dependent on the proteins residing in the B cell surfaceome and their respective nanoscale organization. Here we show that CD20 is a gatekeeper for B cell identity and function due to its control of the functional nanoscale organization of receptors within the B surfaceome and the resting state of B lymphocytes. CRISPR/Cas-based ablation of CD20 in Ramos B cells revealed that IgM class B cell antigen receptor (IgM-BCR) and the co-receptor CD19 are functionally interlinked in the absence of CD20. The resulting IgM-BCR/CD19 signalling synapse leads to transient B cell activation and the loss of B cell identity. Re-expression of CD20 involving an aptamer-controlled riboswitch restores resting-state B cell nanoscale organization and function. Treatment of naive human B cells with the anti-CD20 antibody Rituximab leads to transient B cell activation and formation of transiently activated IgM-BCR/CD19 signalling synapses providing new insights into the molecular mode of action of Rituximab. The loss of B cell identity due to CD20-deficiency is accompanied by a PAX5 to BLIMP-1 transcriptional switch and increased plasma cell development. This cellular B cell differentiation towards plasma B cells is mechanistically accompanied by a metabolic shift towards oxidative phosphorylation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-06-22 |
| AnnouncementXML | Submission_2022-06-22_05:17:37.019.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jonas B Albinus |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Acetyl; Carbamidomethyl; Deamidated |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-06-18 06:44:58 | ID requested | |
| ⏵ 1 | 2022-06-22 05:17:37 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: CD20, B cell, surfaceome |
Contact List
| Bernd Wollscheid | |
|---|---|
| contact affiliation | Institute of Molecular Systems Biology & Department of Health Sciences and Technology, ETH Zurich, Switzerland |
| contact email | bernd.wollscheid@hest.ethz.ch |
| lab head | |
| Jonas B Albinus | |
| contact affiliation | ETH Zuirch |
| contact email | jalbinus@ethz.ch |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085605/ |
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