PXD019873

PXD019873 is an original dataset announced via ProteomeXchange.

Title	Molecular analyses of cotton (Gossypium hirsutum) identifies differential sensitivity to heat during stages of pollen development
Description	Heatwaves resulting from global warming are a leading threat to plants because they specifically impair reproductive (gametophytic) development. We investigated pollen development using proteomics after exposure to heat (38/28 °C; day/night). Sampling enabled the identification of the impact of heat on gene products in tetrads (immediately post-meiosis), uninucleate and binucleate microspores (early mitosis), and mature pollen. A library consisting of 5257 G. hirsutum proteins was constructed using SWATH-MS, which led to quantification of 4501 proteins at the four distinct stages. Data analysis revealed that 880, 360, 307 and 166 proteins were differentially expressed when the tetrad, uninucleate, binucleate and mature pollen stages, respectively, were subjected to heat. Surprisingly, most of these DEPs were identified only at a single developmental stage, indicating translation of stage-specific proteins in response to heat. Especially, high numbers of differentially expressed proteins (DEPs) were identified in tetrads compared with the late developmental stages; these were mainly associated with protein processing in endoplasmic reticulum, oxidative phosphorylation, spliceosome, cytoskeletal response, chaperones and folding catalysts, carbon metabolism and membrane trafficking. We conclude that protein synthesis in tetrad cells responded acutely to heat by supplying cellular components that enable subsequent mitosis and structural changes in the haploid germ cells leading the mature pollen. We hypothesise that fewer DEPs at the later stages might reflect a lower sensitivity to high temperatures. Identifying the stage-specific proteins will lead to the identity of key heat-responsive genes and new genetic tools for improved resilience of all seed and fruit-based crops as climates keep warming.
HostingRepository	PRIDE
AnnounceDate	2021-12-01
AnnouncementXML	Submission_2021-12-20_08:39:22.877.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farhad Masoomi-Aladizgeh
SpeciesList	scientific name: Gossypium hirsutum; NCBI TaxID: 3635;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-18 02:21:17	ID requested
1	2021-12-01 08:20:05	announced
⏵ 2	2021-12-20 08:39:24	announced	2021-12-20: Updated publication reference for PubMed record(s): 34837283.

Masoomi-Aladizgeh F, McKay MJ, Asar Y, Haynes PA, Atwell BJ, Patterns of gene expression in pollen of cotton (Gossypium hirsutum) indicate downregulation as a feature of thermotolerance. Plant J, 109(4):965-979(2022) [pubmed]

submitter keyword: Cotton, Binucleate microspores, Uninucleate microspores, Tetrads, Anthesis, SWATH, Heat stress

Brian J. Atwell
contact affiliation	Department of Biological Sciences at Macquarie University
contact email	brian.atwell@mq.edu.au
lab head
Farhad Masoomi-Aladizgeh
contact affiliation	Macquarie University
contact email	farhad.masoomi-aladizgeh@hdr.mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019873
     1. Label: PRIDE project
     2. Name: Molecular analyses of cotton (Gossypium hirsutum) identifies differential sensitivity to heat during stages of pollen development