PXD019808

PXD019808 is an original dataset announced via ProteomeXchange.

Title	Receptor-independent modulation of cAMP-dependent protein kinase and protein phosphatase 2A signaling in cardiac myocytes by nitroxyl
Description	Sympathetic activation is the main driver of cardiac inotropy and lusitropy. Stimulation of the -adrenoceptor (AR) signaling cascade leads to activation of cAMP-dependent protein kinase (PKA) and subsequent cardiac protein phosphorylation, which is counteracted by the corresponding protein phosphatases 2A (PP2A). Both, kinase and phosphatase are sensitive to oxidation. For PKA, the oxidant-mediated mode of regulation has been described to occur by interdisulfide formation between type I regulatory subunits (PKA-RI). Furthermore, oxidant-mediated intradisulfide formation in the catalytic subunit of PKA and PP2A was shown to inhibit kinase or phosphatase activity, respectively. Nitroxyl donors exert positive inotropic and lusitropic effects in cardiac myocytes.
HostingRepository	PRIDE
AnnounceDate	2020-10-29
AnnouncementXML	Submission_2020-10-29_05:43:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019808
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christoph Krisp
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-16 08:30:26	ID requested
⏵ 1	2020-10-29 05:43:52	announced

Diering S, Stathopoulou K, Goetz M, Rathjens L, Harder S, Piasecki A, Raabe J, Schulz S, Brandt M, Pflaumenbaum J, Fuchs U, Donzelli S, Sadayappan S, Nikolaev VO, Flenner F, Ehler E, Cuello F, Receptor-independent modulation of cAMP-dependent protein kinase and protein phosphatase signaling in cardiac myocytes by oxidizing agents. J Biol Chem, 295(45):15342-15365(2020) [pubmed]

submitter keyword: Rat, Nitroxyl, oxidation, phosphorylation, kinase, phosphatase, cardiac myocyte

Friederike Cuello
contact affiliation	Institute of Experimental Pharmacology and Toxicology; University Medical Center Hamburg-Eppendorf
contact email	f.cuello@uke.de
lab head
Christoph Krisp
contact affiliation	University Medical Center Hamburg-Eppendorf Institute of Clinical Chemistry and Laboratory Medicine Mass Spectrometric Proteomics
contact email	c.krisp@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD019808

PRIDE project URI

• PRIDE
  1. PXD019808
     1. Label: PRIDE project
     2. Name: Receptor-independent modulation of cAMP-dependent protein kinase and protein phosphatase 2A signaling in cardiac myocytes by nitroxyl