PXD019807

PXD019807 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteomic analysis provides insights into the aluminum-responsive in Tamba black soybean
Description	Aluminum (Al) toxicity is one of the most important limitations to agricultural production worldwide. The overall response of plants to Al stress has been documented, but the contribution of protein phosphorylation to Al detoxicity and tolerance in plants is unclear. Using a combination of tandem mass tag (TMT) labelling, immobilized metal affinity chromatography (IMAC) enrichment and liquid chromatography-mass spectrometry /mass spectrometry (LC-MS/MS), Al-induced phosphoproteomic changes in roots of Tamba black soybean (TBS) was investigated in this study. After AlCl3 treatment, 314 proteins harbouring 420 phosphosites, were significantly changed (fold change > 1.2 or < 0.83, p < 0.05) with 151 up-regulated, 157 down-regulated and 6 up/down-regulated. Enrichment and protein interaction analyses revealed that differentially phosphorylated proteins (DPPs) under Al treatment was mainly related to G-mediated signaling, Ca2+, transcription, translation, transporters and carbohydrate metabolism, roughly clustered for one of associated with inhibition to root growth, and another of citric acid production for resistance to Al detoxicity, which was facilitated by Al3+ through modifying phosphorylation of proteins responsible for carbohydrate metabolism. The results provide novel insights into the molecular mechanisms of TBS post-translational modifications in response to Al stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:23.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rongrong Han
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-16 08:30:23	ID requested
1	2020-08-27 00:24:52	announced
⏵ 2	2024-10-22 05:11:26	announced	2024-10-22: Updated project metadata.

10.1371/journal.pone.0237845;

Han R, Wei Y, Xie Y, Liu L, Jiang C, Yu Y, Quantitative phosphoproteomic analysis provides insights into the aluminum-responsiveness of Tamba black soybean. PLoS One, 15(8):e0237845(2020) [pubmed]

submitter keyword: Phosphoproteome

aluminum stress

Tamba black soybean

TMT labelling

roots tips

Yongxiong Yu
contact affiliation	Southwest University
contact email	yuyongxiong8@126.com
lab head
Rongrong Han
contact affiliation	College of Animal Science and Technology, Southwest University
contact email	19891214rong@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019807
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteomic analysis provides insights into the aluminum-responsive in Tamba black soybean