PXD019803

PXD019803 is an original dataset announced via ProteomeXchange.

Title	iotin-streptavidin affinity purification mass spectrometry of proteins in human 293T cell using bioinylated Orf9b
Description	COVID-19, caused by the new RNA virus strain SARS-CoV-2, is a serious acute respiratory disease. One of the prominent feature of SARS-COV-2 in terms of immune responses is that it triggers very low level responses of IFN-I in cells or COVID-19 patients. Previously SARS-CoV Orf9b were reported to be involved in inhibiting the production of IFN-I by targeting mitochondria. To understand the molecular underpinning of SARS-CoV-2 Orf9b in the host cell, we employed a biotin-streptavidin affinity purification mass spectrometry (MS) approach to identify the human proteins that physically interact with Orf9b.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:57:22.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingfeng Meng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-16 03:47:26	ID requested
⏵ 1	2021-09-09 00:57:23	announced

Jiang HW, Zhang HN, Meng QF, Xie J, Li Y, Chen H, Zheng YX, Wang XN, Qi H, Zhang J, Wang PH, Han ZG, Tao SC, SARS-CoV-2 Orf9b suppresses type I interferon responses by targeting TOM70. Cell Mol Immunol, 17(9):998-1000(2020) [pubmed]

submitter keyword: SILAC, Human 293T cell, SARS-CoV-2 Orf9b, AP-MS

Sheng-ce Tao
contact affiliation	Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China
contact email	taosc@sjtu.edu.cn
lab head
Qingfeng Meng
contact affiliation	Shanghai Jiao Tong University
contact email	mengqf3321@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019803

PRIDE project URI

• PRIDE
  1. PXD019803
     1. Label: PRIDE project
     2. Name: iotin-streptavidin affinity purification mass spectrometry of proteins in human 293T cell using bioinylated Orf9b