PXD019802

PXD019802 is an original dataset announced via ProteomeXchange.

Title	Sulfide Regulates Autophagy by Reversible Persulfidation of the Cysteine Protease ATG4
Description	Hydrogen sulfide is a signaling molecule that regulates essential processes for plant performance, such as autophagy. In Arabidopsis thaliana, hydrogen sulfide negatively regulates autophagy independently of reactive oxygen species, but the underlying mechanism remains to be understood. To determine whether persulfidation, an emergent posttranslational modification, has a main role in the control of autophagy by sulfide, we have used a proteomic approach targeting ATG4, a cysteine protease that plays a crucial role in autophagy progression. We showed that AtATG4a from A. thaliana, which is the predominant ATG4 protease in this plant species, contains a specific site for persulfidation, the residue Cys170, included in the characteristic catalytic triad Cys-His-Asp of cysteine proteases. We tested whether persulfidation regulates the activity of ATG4 by setting up a heterologous assay using the Chlamydomonas reinhardtii CrATG8 protein as a substrate. Our findings demonstrate that sulfide significantly inactivates AtATG4a cleavage activity in a reversible manner. The biological significance of the reversible inhibition of the ATG4 protease by sulfide is supported by our findings in Arabidopsis leaves under both basal and autophagy-inducing conditions. We also observed a significant increase in the overall ATG4 activity in Arabidopsis under nitrogen starvation and osmotic stress, which is inhibited by sulfide. Therefore, our data strongly suggest that the negative regulation of autophagy by sulfide is mediated, at least, by the specific persulfidation of the protease ATG4.
HostingRepository	PRIDE
AnnounceDate	2020-12-14
AnnouncementXML	Submission_2020-12-14_00:09:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luis C. Romero
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	methylthiolated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-16 03:42:41	ID requested
⏵ 1	2020-12-14 00:09:14	announced

Laureano-Mar, í, n AM, Aroca Á, P, é, rez-P, é, rez ME, Yruela I, Jurado-Flores A, Moreno I, Crespo JL, Romero LC, Gotor C, Abscisic Acid-Triggered Persulfidation of the Cys Protease ATG4 Mediates Regulation of Autophagy by Sulfide. Plant Cell, 32(12):3902-3920(2020) [pubmed]

submitter keyword: Arabidopsis, abscisic acid, hydrogen sulfide, persulfidation

Luis C Romero
contact affiliation	Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Seville, Spain
contact email	lromero@ibvf.csic.es
lab head
Luis C. Romero
contact affiliation	Instituto Bioquimica Vegetal y Fotosintesis-CSIC
contact email	lromero@ibvf.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019802
     1. Label: PRIDE project
     2. Name: Sulfide Regulates Autophagy by Reversible Persulfidation of the Cysteine Protease ATG4