PXD019799

PXD019799 is an original dataset announced via ProteomeXchange.

Title	Aggregated protein of heat-treated GmDnj1 mutant Orbitrap LC-MSMS
Description	Gmdnj1-knockout mutants had diminished growth in normal conditions, and when under heat stress, exhibited more severe browning, reduced chlorophyll contents, higher reactive oxygen species (ROS) contents, and higher induction of heat stress-responsive transcription factors and ROS-scavenging enzyme-encoding genes. Knocking out a major HSP40 may lead to the impairment of the protein refolding system, and in turn lead to a change in the aggregated protein profile. Therefore, the aggregated protein profiles of the Gmdnj1 mutant lines were compared against that of the wild type Williams 82 under both untreated and heat-treated conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:33.871.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019799
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kwan Pok Li
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-16 00:50:51	ID requested
1	2021-09-09 03:05:18	announced
⏵ 2	2024-10-22 05:28:36	announced	2024-10-22: Updated project metadata.

Li KP, Wong CH, Cheng CC, Cheng SS, Li MW, Mansveld S, Bergsma A, Huang T, van Eijk MJT, Lam HM, GmDNJ1, a type-I heat shock protein 40 (HSP40), is responsible for both Growth and heat tolerance in soybean. Plant Direct, 5(1):e00298(2021) [pubmed]

10.6019/PXD019799;

10.1002/pld3.298;

submitter keyword: aggregated protein, GmDNJ1, heat stress, soybean

Hon-Ming Lam
contact affiliation	School of Life Sciences and Center for Soybean Research of the State Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong SAR
contact email	honming@cuhk.edu.hk
lab head
Kwan Pok Li
contact affiliation	The Chinese University of Hong Kong
contact email	1155089709@link.cuhk.edu.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019799

PRIDE project URI

• PRIDE
  1. PXD019799
     1. Label: PRIDE project
     2. Name: Aggregated protein of heat-treated GmDnj1 mutant Orbitrap LC-MSMS