PXD019789

PXD019789 is an original dataset announced via ProteomeXchange.

Title	Root isoprene formation alters lateral root development Maja Miloradovic van Doorn
Description	Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analyzing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O2 - in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signaling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signaling network and development processes in root tissue. This article is protected by copyright. All rights reserved.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:18.746.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ann-Christine König
SpeciesList	scientific name: Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa); NCBI TaxID: 3694;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-15 07:28:21	ID requested
1	2020-06-16 03:01:34	announced
⏵ 2	2024-10-22 05:07:26	announced	2024-10-22: Updated project metadata.

10.1111/pce.13814;

Miloradovic van Doorn M, Merl-Pham J, Ghirardo A, Fink S, Polle A, Schnitzler JP, Rosenkranz M, Root isoprene formation alters lateral root development. Plant Cell Environ, 43(9):2207-2223(2020) [pubmed]

submitter keyword: Populus x canescens

ROS

VOC

isoprene

isoprene promoter

lateral roots

poplar

reactive oxygen species

roots

volatile organic compounds.

Prof. Dr. Jörg-Peter Schnitzler
contact affiliation	Helmholtz Zentrum München, Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany
contact email	jp.schnitzler@helmholtz-muenchen.de
lab head
Ann-Christine König
contact affiliation	HMGU Core Facility Proteomics
contact email	ann-christine.koenig@helmholtz-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019789
     1. Label: PRIDE project
     2. Name: Root isoprene formation alters lateral root development Maja Miloradovic van Doorn