⮝ Full datasets listing

PXD019789

PXD019789 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRoot isoprene formation alters lateral root development Maja Miloradovic van Doorn
DescriptionIsoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analyzing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O2 - in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signaling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signaling network and development processes in root tissue. This article is protected by copyright. All rights reserved.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:07:18.746.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnn-Christine König
SpeciesList scientific name: Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa); NCBI TaxID: 3694;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-15 07:28:21ID requested
12020-06-16 03:01:34announced
22024-10-22 05:07:26announced2024-10-22: Updated project metadata.
Publication List
10.1111/pce.13814;
Miloradovic van Doorn M, Merl-Pham J, Ghirardo A, Fink S, Polle A, Schnitzler JP, Rosenkranz M, Root isoprene formation alters lateral root development. Plant Cell Environ, 43(9):2207-2223(2020) [pubmed]
Keyword List
submitter keyword: Populus x canescens
ROS
VOC
isoprene
isoprene promoter
lateral roots
poplar
reactive oxygen species
roots
volatile organic compounds.
Contact List
Prof. Dr. Jörg-Peter Schnitzler
contact affiliationHelmholtz Zentrum München, Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany
contact emailjp.schnitzler@helmholtz-muenchen.de
lab head
Ann-Christine König
contact affiliationHMGU Core Facility Proteomics
contact emailann-christine.koenig@helmholtz-muenchen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019789
PRIDE project URI
Repository Record List
[ + ]