PXD019789 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Root isoprene formation alters lateral root development Maja Miloradovic van Doorn |
Description | Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analyzing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O2 - in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signaling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signaling network and development processes in root tissue. This article is protected by copyright. All rights reserved. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:07:18.746.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ann-Christine König |
SpeciesList | scientific name: Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa); NCBI TaxID: 3694; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-15 07:28:21 | ID requested | |
1 | 2020-06-16 03:01:34 | announced | |
⏵ 2 | 2024-10-22 05:07:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1111/pce.13814; |
Miloradovic van Doorn M, Merl-Pham J, Ghirardo A, Fink S, Polle A, Schnitzler JP, Rosenkranz M, Root isoprene formation alters lateral root development. Plant Cell Environ, 43(9):2207-2223(2020) [pubmed] |
Keyword List
submitter keyword: Populus x canescens |
ROS |
VOC |
isoprene |
isoprene promoter |
lateral roots |
poplar |
reactive oxygen species |
roots |
volatile organic compounds. |
Contact List
Prof. Dr. Jörg-Peter Schnitzler |
contact affiliation | Helmholtz Zentrum München, Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany |
contact email | jp.schnitzler@helmholtz-muenchen.de |
lab head | |
Ann-Christine König |
contact affiliation | HMGU Core Facility Proteomics |
contact email | ann-christine.koenig@helmholtz-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019789 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019789
- Label: PRIDE project
- Name: Root isoprene formation alters lateral root development Maja Miloradovic van Doorn