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PXD019776

PXD019776 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOptimisation of sporosori purification and protein extraction techniques for the biotrophic protozoan plant pathogen Spongospora subterranea
DescriptionSpongospora subterranea is a soil-borne plant pathogen responsible for economically significant root and powdery scab diseases of potato. However, Tthe obligate biotrophic nature of S. subterranea has made detailed study of the pathogen problematic. Here, we first compared the benefits of sporosori partial purification utilizing Ludox gradient centrifugation. We then undertook optimization efforts for protein isolation comparing the use of a urea buffer followed by Single-Pot Solid-Phase-enhanced Sample Preparation (SP3) and a sodium dodecyl sulfate (SDS) buffer followed by suspension-trapping (S-Trap). Label-free, quantitative proteomics was then used to evaluate the efficiency of sporosori purification and protein preparation methods. The purification protocol produced a highly purified suspension of S. subterranea sporosori without affecting the viability of the spores. Results indicated that the use of a combination of SDS and S-Trap for sample clean-up and digestion obtained a significantly higher number of identified proteins compare to using urea and SP3, with 218 and 652 proteins identified using SP3 and S-Trap methods, respectively. Analysis of proteins by mass spectrometry showed that the number of identified proteins increased by approximately 40% after the purification of spores by Ludox. These results suggested a potential use of the described spore purification and protein preparation methods for proteomics study of obligate biotrophic pathogens such as S. subterranea.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:32:00.320.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRichard Wilson
SpeciesList scientific name: Spongospora sp.; NCBI TaxID: 1979856;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-15 02:50:40ID requested
12022-02-15 13:11:16announced
22024-10-22 05:32:01announced2024-10-22: Updated project metadata.
Publication List
Balotf S, Wilson R, Tegg RS, Nichols DS, Wilson CR, . Molecules, 25(14):(2020) [pubmed]
10.3390/molecules25143109;
Keyword List
submitter keyword: Spongospora subterranea
sporosori
density gradient centrifugation
Ludox
proteomics
S-Trap.
Contact List
Dr Richard Wilson
contact affiliationProteomics facility, Central Science Laboratory, University of Tasmania
contact emailrichard.wilson@utas.edu.au
lab head
Richard Wilson
contact affiliationUniversity of Tasmania
contact emailrichard.wilson@utas.edu.au
dataset submitter
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Dataset FTP location
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