PXD019765

PXD019765 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Arabidopsis plastoglobules 2D-gel separation
Description	Protein complexes separation of isolated plastoglobules (PGs) from Arabidopsis thaliana (Col-0 ecotype). PGs were isolated from the plants when they were 4 weeks old under long day photoperiod ( during the morning in dark adapted plants,16 hours of light and 8 darkness photoperiod, LED light of 120 uE intensity). The PGs were solubilized with 1.1 % n-Dodecyl--D-Maltoside and separated in a Blue-Native gel. The gel lanes were excised and further separated in a denaturing second dimension by SDS-PAGE. The resulting gel was visualized by silver staining and the protein spots excised for in-gel trypsin digestion. Samples from the 2D gel were subjeted to shotgun proteomics. The protein samples were measured on an LTQ Orbitrap (Thermo Fisher Scientific Inc, Waltham Massachusetts, USA) coupled to a Rheos Allegro liquid chromatograph (Flux Instruments GmbH, Klaus Kaiser Hochstr. 48, 4002 Basel, CH).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:21:05.798.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Espinoza Corral
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Classic

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-15 02:49:08	ID requested
1	2021-04-13 12:08:22	announced
⏵ 2	2024-10-22 05:21:10	announced	2024-10-22: Updated project metadata.

Publication List

Espinoza-Corral R, Schwenkert S, Lundquist PK, Molecular changes of Arabidopsis thaliana plastoglobules facilitate thylakoid membrane remodeling under high light stress. Plant J, 106(6):1571-1587(2021) [pubmed]
10.1111/tpj.15253;

Espinoza-Corral R, Schwenkert S, Lundquist PK, Molecular changes of Arabidopsis thaliana plastoglobules facilitate thylakoid membrane remodeling under high light stress. Plant J, 106(6):1571-1587(2021) [pubmed]

10.1111/tpj.15253;

Keyword List

submitter keyword: PGs,Arabidopsis

submitter keyword: PGs,Arabidopsis

Contact List

Peter K. Lundquist
contact affiliation	Plant & Soil Science Building 1066 Bogue St, Rm A494E East Lansing, MI 48824 Michigan State University, United states.
contact email	pklundqu@msu.edu
lab head
Roberto Espinoza Corral
contact affiliation	Michigan State University
contact email	espino95@msu.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD019765
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD019765

PRIDE project URI

Repository Record List

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