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PXD019755

PXD019755 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLkb1 suppresses amino acid-driven gluconeogenesis in the liver
DescriptionExcessive glucose production in the liver is a key factor in the hyperglycemia observed in diabetes mellitus type 2. It is generally agreed to result from an increase in hepatic gluconeogenesis. Considerable attention has been devoted to the transcriptional regulation of key gluconeogenic enzymes, but much less is known about the regulation of amino-acid catabolism, which generates gluconeogenic substrates. Here, we highlight a novel role of LKB1 in this regulation. We show that mice with a hepatocyte-specific deletion of Lkb1 have higher levels of hepatic amino acid catabolism, driving gluconeogenesis. This effect was observed during both fasting and the postprandial period, identifying Lkb1 as a critical suppressor of postprandial hepatic gluconeogenesis. Hepatic Lkb1 deletion was associated with major changes in whole-body metabolism, leading to a lower lean body mass and, in the longer term, sarcopenia and cachexia, as a consequence of the diversion of amino acids to liver metabolism at the expense of muscle. Using genetic and pharmacological approaches, we identified the aminotransferases and specifically, Agxt as effectors of the suppressor function of Lkb1 in amino acid-driven gluconeogenesis. The present dataset is from the phosphoproteomic analysis of the refed mice in a study where a global quantitative analysis ( PXD013478 ) is also described in the same publication.
HostingRepositoryPRIDE
AnnounceDate2020-12-01
AnnouncementXMLSubmission_2020-11-30_23:39:40.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrançois GUILLONNEAU
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-15 02:47:57ID requested
12020-11-30 23:39:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LKB1, gluconeogenesis, liver, amino acids, sarcopenia, cachexia, orbitrap Fusion, Q-exactive, phosphoproteome, label-free quantification, refed mice
Contact List
Christine PERRET
contact affiliationInserm U1016, Institut Cochin, 24 rue du Faubourg Saint-Jacques, 75014 PARIS, FRANCE.
contact emailchristine.perret@inserm.fr
lab head
François GUILLONNEAU
contact affiliation3P5 Université Paris Descartes
contact emailfrancois.guillonneau@parisdescartes.fr
dataset submitter
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Dataset FTP location
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