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PXD019754

PXD019754 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19 complex in meiotic kinetochore assembly.
DescriptionFaithful gamete formation during meiosis requires that kinetochores take on new functions that impact homolog pairing/recombination and their attachments to microtubules. We used a proteomics pipeline to determine the global composition of centromeric chromatin and kinetochores at distinct meiotic stages. Our datasets uncover an unanticipated role for the Ctf19CCAN inner kinetochore sub-complex in gametogenesis. We show that components of the Ctf19 complex that are dispensable for mitotic growth become critical for assembly of a functional kinetochore upon meiotic entry. Consequently, in the absence of these factors, chromosomes fail to attach to meiotic spindles and produce inviable gametes due to gross segregation errors. Our evidence suggests that functional kinetochore assembly is driven by Ctf19CCAN complex-dependent Ipl1AURORA B positioning at the inner kinetochore. Thus, our global view of kinetochore composition in meiosis reveals the extent of kinetochore remodelling during gametogenesis and uncovers a kinetochore assembly pathway that becomes critical in meiosis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:19:15.062.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdele Marston
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-15 02:47:52ID requested
12021-02-23 09:49:06announced
22024-10-22 05:19:18announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.cub.2020.10.025;
Borek WE, Vincenten N, Duro E, Makrantoni V, Spanos C, Sarangapani KK, de Lima Alves F, Kelly DA, Asbury CL, Rappsilber J, Marston AL, Complex in Meiotic Kinetochore Assembly. Curr Biol, 31(2):283-296.e7(2021) [pubmed]
Keyword List
submitter keyword: kinetochores, Saccharomyces cerevisiae,Meiosis, chromatin, label-free quantitation.
Contact List
Adele Marston
contact affiliationWCCB, University of Edinburgh
contact emailadele.marston@ed.ac.uk
lab head
Adele Marston
contact affiliationUniversity of Edinburgh
contact emailadele.marston@ed.ac.uk
dataset submitter
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Dataset FTP location
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Repository Record List
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